深圳市男男性行为人群艾滋病病毒主要流行亚型的分子网络特征分析  

Molecular network characteristics of three main subtypes of HIV among MSM in Shenzhen

作　　者：黄元妹 袁苑 郑陈丽[2] 李浩[2] 朱悦 李思美 张燕[2] 谭唯[2] 赵锦 HUANG Yuan-mei;YUAN Yuan;ZHENG Chenli;LI Hao;ZHU Yue;LI Simei;ZHANG Yan;TAN Wei;ZHAO Jin(School of Public Health,Shanxi Medical University,Taiyuan 030001,China;不详)

机构地区：[1]山西医科大学公共卫生学院,太原030001 [2]深圳市疾病预防控制中心,广东深圳518000 [3]汕头大学医学院,广东汕头515014 [4]中山大学公共卫生学院,广州510080

出　　处：《实用医学杂志》2022年第21期2745-2750,共6页The Journal of Practical Medicine

基　　金：广东省基础与应用基础研究基金(编号:2019B1515120003);深圳市医疗卫生三名工程(编号:SZSM201811071)。

摘　　要：目的了解深圳市艾滋病病毒(HIV)感染者中男男性行为人群(men who have sex with men,MSM)三种主要亚型分子网络特征,为控制MSM HIV的传播提供线索。方法收集深圳市2017至2019年新报告MSM HIV感染者资料和血样,使用在线工具和Mega分析亚型、HyPhy计算成对基因距离、Cytoscape实现分子网络可视化及SPSS进行统计分析。结果获得2848名MSM HIV感染者pol区基因序列,分为11种亚型,其中CRF07_BC、CRF01_AE、CRF55_01B占91.22%。三种亚型总入网率为32.32%,形成194个簇和1291条边。入网的MSM HIV感染者主要集中在35岁以下、汉族、未婚、外省或外籍、商业服务、无性病史、201~500个/μL CD4^(+)T细胞,CRF01_AE感染者主要集中在高中或中专、CRF07_BC感染者主要集中在大专及以上、CRF55_01B感染者主要集中在初中及以下。CRF01_AE感染者中,201~500个/μL CD4^(+)T细胞的入网率最高;CRF07_BC感染者易发生大簇聚集;CRF55_01B感染者中,初中及小学学历的入网率最高。结论CRF01_AE感染者中,201~500个/μL CD4^(+)T细胞数传播风险最高,CRF07_BC大簇中感染者传播风险较高,CRF55_01B感染者中,初中及小学的传播风险最高。Objective To investigate the molecular network characteristics of three major subtypes among HIV positive men who have sex with men(MSM)in Shenzhen in order to provide clues for controlling the transmis⁃sion of HIV.Methods Data and blood samples were collected from newly reported MSM who infected with HIV in Shenzhen from 2017 to 2019.Subtype analysis was performed by online tools and Mega software.Pairwise genetic distances were calculated by HyPhy.Molecular network visualization was constructed by Cytoscape,and statistical data analysis was used by SPSS software.Result A total of 2848 genetic sequences of pol region of HIV were obtained from newly reported HIV positive MSM and successfully classified into 11 subtypes,of which three major subtypes including CRF07_BC,CRF01_AE,CRF55_01B accounted for 91.22%.The overall access rate of the three main subtypes was 32.32%,forming 194 clusters and 1291 edges.Entering the molecular network,MSM infected with HIV were mainly under 35 years old,Han nationality,unmarried,from other provinces or foreign countries,commercial service,no sexual history,201~500 CD4^(+)T cells/μL,MSM infected with CRF01_AE mainly concen⁃trated in high school or technical secondary school,MSM infected with CRF07_BC mainly concentrated in junior col⁃lege or above,and MSM infected with CRF55_01B mainly concentrated in junior high school or below.MSM infected with CRF01_AE,the access rate of the group of CD4 counts with 201⁃500 cells/μL was the highest.MSM infected with CRF07_BC were prone to forming large clusters.MSM infected by CRF55_01B had the highest access rate in junior high school and primary school.Conclusion MSM infected with CRF01_AE,the transmission risk of CD4^(+)T cells was highest in the range of 201~500/μL,and the transmission risk was higher in CRF07_BC clusters.MSM infected with CRF55_01B,the transmission risk was highest in junior high schools and primary schools.

关 键 词：艾滋病病毒 男男性行为人群 分子网络 

分 类 号：R181.1[医药卫生—流行病学] R512.91[医药卫生—公共卫生与预防医学]