不同类型植物病原物中分泌蛋白及CAZymes对比研究  

Comparative Study of Secretory Proteins and CAZymes in Different Types of Plant Pathogens

作　　者：夏雄飞 陈云芳 覃悦 韩长志[1,3] XIA Xiongfei;CHEN Yunfang;QIN Yue;HAN Changzhi(College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,China;Suzhou Customs of the People’s Republic of China,Suzhou 215000,China;Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Kunming 650224,China)

机构地区：[1]西南林业大学生物多样性保护学院,云南昆明650224 [2]中华人民共和国苏州海关,江苏苏州215000 [3]云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《云南农业大学学报（自然科学版）》2022年第6期949-956,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31960314);云南省高层次人才培养支持计划“青年拔尖人才”专项(YNWR-QNBJ-2020-188);云南省应用基础研究计划项目(2018FG001-028)。

摘　　要：【目的】通过对不同类型植物病原物中分泌蛋白及碳水化合物活性酶(CAZymes)进行系统对比分析,为深入解析分泌蛋白及CAZymes在不同植物病原物致病机制中的重要作用提供理论基础。【方法】选择9种真菌、5种细菌和9种卵菌共23种不同类型病原物作为研究对象,根据分泌蛋白的基本特征,采用SignalP v5.0等在线分析程序对病原物中的分泌蛋白进行预测;利用在线工具dbCAN对获取的分泌蛋白进行CAZymes预测;最终对3种类型植物病原物预测结果进行系统对比分析。【结果】真菌、细菌和卵菌的分泌蛋白数量分别为71~510、105~227和119~768个,所占比例分别为0.74%~3.93%、2.05%~3.91%和0.96%~2.90%;真菌、细菌和卵菌中含有的CAZymes平均数量分别为26、4和26个,种类分别为62~76、8~14和24~36种,其中真菌中AA9、GH11和GH28等类别的CAZymes数量较多,细菌中GH25和PL_6的数量较多,卵菌中PL3_2和GH17的数量较多。【结论】卵菌的分泌蛋白占比较小,真菌的分泌蛋白占比最值差异较大;细菌的CAZymes数量与种类最少,真菌的CAZymes种类最多,显示了不同类型植物病原物与植物互作以及种间竞争等协同进化过程中存在共性化和差异化的毒性策略。[Purpose]Through systematic comparative analysis of secretory proteins and carbohydrate-active enzymes(CAZymes)in different types of plant pathogens,this study provides a theoretical basis for in-depth analysis of the important roles of secretory proteins and CAZymes in the pathogenesis of different plant pathogens.[Methods]A total of 23 pathogens of different types,in-cluding nine fungi,five bacteria and nine oomycetes,were selected as research objects.According to the basic characteristics of secretory proteins,online analysis programs such as SignalP v5.0 were used to predict secretory proteins in pathogens;CAZymes prediction of secretory proteins was per-formed by online tool dbCAN.Finally,the prediction results of three kinds of plant pathogens were compared systematically.[Results]The number of secretory proteins in fungi ranged from 71 to 510,accounting for 0.74%to 3.93%,the number of secretory proteins in bacteria ranged from 105 to 227,accounting for 2.05%to 3.91%,and the number of secretory proteins in oomycetes ranged from 119 to 768,accounting for 0.96%to 2.90%.The average number of CAZymes in fungi,bacteria and oomycetes was 26,4 and 26,respectively,and there were 62-76,8-14 and 24-36 kinds of CAZymes,respectively.Among them,fungi had more CAZymes of AA9,GH11 and GH28;bacteria had more GH25 and PL_6,while oomycetes had more PL3_2 and GH17.[Conclusion]The proportion of secretory proteins in oomycetes is relatively small,and the maximum proportion of secretory proteins in fungi varies greatly.The number and kinds of CAZymes in bacteria were the least,while the kinds of CAZymes in fungi were the most.The study indicated that there were common and differentiated toxicity strategies in the coevolution process of different plant pathogens interacting with plants and competing among species.

关 键 词：植物病原物 分泌蛋白 CAZymes 生物信息学 

分 类 号：S432.23[农业科学—植物病理学]