HT29和HCT116结直肠癌细胞增殖及其裸鼠移植成瘤研究  

Cell Proliferation of HT29 and HCT116 Colorectal Cancer Cell and Their Tumor Formation in Nude Mice

作　　者：王霞 朱飞艳 王晶 熊喆 韦云芳 李彬 魏红江 赵红业 WANG Xia;ZHU Feiyan;WANG Jing;XIONG Zhe;WEI Yunfang;LI Bin;WEI Hongjiang;ZHAO Hongye(College of Pharmaceutical Science,Dali University,Dali 671000,China;Key Laboratory of Animal Gene Edit-ing and Animal Cell Cloning in Yunnan Province,Kunming 650201,China;Xenotransplantation Research Engineering Center in Yunnan Province,Kunming 650201,China;College of Animal Medicine,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]大理大学药学与化学学院,云南大理671000 [2]云南省动物基因编辑与体细胞克隆技术重点实验室,云南昆明650201 [3]云南省异种器官移植工程研究中心,云南昆明650201 [4]云南农业大学动物医学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2022年第6期985-991,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(81872452)。

摘　　要：【目的】比较HT29和HCT116两株细胞系体外培养细胞增殖和在裸鼠体内移植后的成瘤情况。【方法】检测结直肠癌细胞HT29和HCT116的细胞活力,观察克隆形成细胞的形态,检测2株细胞p53和p21蛋白的表达水平;将2株细胞移植于裸鼠右侧腹股沟皮下,观察移植瘤的生长情况,记录裸鼠的体质量及移植瘤的体积。【结果】与HT29细胞相比,HCT116细胞在体外的增长率极显著升高(P<0.01),p21蛋白正常表达,p53蛋白表达水平极显著降低(P<0.01);在阿霉素存在的情况下,HCT116细胞中的p21蛋白表达水平极显著升高(P<0.01)。14 d内HCT116细胞移植量对裸鼠体质量无显著影响(P>0.05);单只细胞接种量为4×10^(5)和2×10^(6)个时,HT29细胞肿瘤体积显著高于HCT116细胞(P<0.05)。【结论】HCT116细胞在体外的增殖速率显著高于HT29细胞,但是在体内HT29细胞比HCT116细胞更易成瘤,其分子基础可能与HT29细胞中p53基因突变导致的p53蛋白高表达及其下游p21蛋白低表达有关;高浓度的癌细胞移植有利于肿瘤形成。本研究为结直肠癌动物模型的建立及其进一步研究奠定了基础。[Purpose]To compare the proliferation of HT29 and HCT116 cell lines cultured in vitro and the tumorigenesis after transplantation in nude mice.[Methods]The viability of colorectal cancer cells HT29 and HCT116 were detected,observing the morphology of clonogenic experimental cells,and detecting the expression levels of p53 and p21 proteins in the two cell lines.The two cell lines were transplanted under the right groin of nude mice,observing the growth of the transplanted tumor,and recording the weight and volume of the transplanted tumor.[Results]Compare with HT29 cells,the proliferation rate of HCT116 cells in vitro was extremely significantly higher(P<0.01),the expression level of p21 protein was normal,but the expression level of p53 protein was extremely significantly lower(P<0.01);in the presence of doxorubicin,the expression level of p21 protein in HCT116 cell line was extremely significantly higher(P<0.01).The number of trans-planted HCT116 cells within 14 days had no significant effect on the body weight of nude mice(P>0.05).Under the amount of inoculation of per mice by 4×10^(5) and 2×10^(6),the tumor volume of nude mice transplanted with HT29 cells was significantly higher than that of HCT116 cells(P<0.05).[Conclusion]In vitro,the proliferation rate of HCT116 cells is significantly higher than that of HT29 cells.However,in vivo,HT29 cells transplanted into nude mice are more likely to form tumors than HCT116 cells.The molecular mechanism may be related to the high expression of p53 protein and the low expression of downstream p21 protein caused by p53 gene mutation in HT29 cells.High-er concentrations of cancer cells are more conducive to tumor formation.This study provides a theor-etical basis for the construction of colorectal cancer animal model and its further application.

关 键 词：结直肠癌 HT29细胞 HCT116细胞 细胞增殖 皮下移植瘤 

分 类 号：S865.19[农业科学—野生动物驯养]