大白菜COBRA基因家族鉴定及其低温胁迫表达分析  

Identification and Expression Analysis under Low Temperature Stress of COBRA Gene Family in Brassica rapa L.

作　　者：廉瑞婷 任宏 翟文 陈连水[2] 汤晓辛[1] LIAN Ruiting;REN Hong;ZHAI Wen;CHEN Lianshui;TANG Xiaoxin(School of Life Sciences,Guizhou Normal University,Guiyang 550025,China;School of Chemistry,Biology and Materials Science,East China University of Technology,Nanchang 330013,China)

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550025 [2]东华理工大学化学生物与材料科学学院,江西南昌330013

出　　处：《云南农业大学学报（自然科学版）》2022年第6期1031-1039,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(U1812401,31560184,31300317,31760076)。

摘　　要：【目的】鉴定大白菜COBRA基因家族成员,分析基因在低温胁迫下的表达并预测基因功能。【方法】利用生物信息学方法对大白菜的COBRA基因家族进行鉴定,并对基因结构、系统进化、启动子元件和表达模式进行分析。【结果】大白菜COBRA基因家族包含23个成员,命名为BrCOBL1~BrCOBL23,分布于大白菜基因组的8条染色体上,根据基因序列特点将其分为2个亚族。大白菜与同属于十字花科的拟南芥、甘蓝型油菜、甘蓝和芥菜基因组中共鉴定出147个COBRA基因,系统进化树分析表明以上COBRA基因家族成员可聚为8个亚组。基因表达分析显示:低温胁迫下,大白菜BrCOBL1、BrCOBL2、BrCOBL15和BrCOBL16的表达量显著升高。【结论】COBRA蛋白序列含有共同的保守基序,且某些基序特异存在于不同的亚族,表明这些基序可能与各亚族的功能分化有关。根据启动子分析结果推测BrCOBL基因可能参与大白菜的非生物胁迫响应及激素应答调控。[Purposes]To identify the members of COBRA gene family in Chinese cabbage(Brassica rapa L.),and the gene expression under low temperature will be analyzed to predict the gene function.[Methods]The COBRA gene family of B.rapa was identified by bioinformatics method,and the gene structure,phylogenetic evolution,promoter elements and expression pattern were analyzed.[Results]The COBRA gene family contained 23 members in B.rapa,named BrCOBL1-BrCOBL23,which were divided into two subfamilies according to the characteristics of gene sequence,and distributed on eight chromosomes.A total of 147 COBRA genes were identified from B.rapa,Arabidopsis thaliana,B.napus,B.oleracea,and B.juncea,which all belonged to Brassicaceae.Phylogenetic tree analysis showed that the members of COBRA gene family were di-vided into eight subgroups.The expression analysis of genes showed that:the expression levels of BrCOBL1,BrCOBL2,BrCOBL15 and BrCOBL16 were significantly increased under low temperat-ure stress.[Conclusion]COBRA protein sequences contain common conserved motifs,and some motifs exist specifically in different subfamilies,indicating that these motifs may be related to the functional differentiation of each subfamily.According to the promoter analysis results,it is speculated that BrCOBL is involved in the regulation of abiotic stress response and hormone response in B.rapa.

关 键 词：大白菜 COBRA基因家族 基因表达分析 低温胁迫 

分 类 号：S634.101[农业科学—蔬菜学]