Isolation and Identification of Squash Leaf Curl China Virus in Zucchini  

作　　者：Huixian SHI Yimin GU Xingsheng LI Xiping SUN Yangdong GUO Xiaowei YUAN 

机构地区：[1]Huasheng Seed Group Co.,Ltd.,Qingzhou 262500,China [2]College of Horticulture,China Agricultural University,Beijing 100193,China

出　　处：《Agricultural Biotechnology》2022年第5期1-3,14,共4页农业生物技术（英文版）

基　　金：Supported by Shandong Province Key R&D Program(2021LZGC015);Taishan Industry Leading Talent Project(LJNY201812)。

摘　　要：[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong Province.Primers were further designed for amplifying the full-length SLCCNV.The full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus disease.Since the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.

关 键 词：ZUCCHINI Viral disease Squash leaf curl China virus 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]