Primary Research on UVB-irradiation Protective Effect of Total Flavonoids from Large-leaved Kuding Tea (Ilex latifolia Thunb.)  

作　　者：Liyan LI Tao HUANG Sen PANG Xiaoning LIU 

机构地区：[1]Medical School,Huanghe Science&Technology,Zhengzhou 450063,China

出　　处：《Agricultural Biotechnology》2022年第5期15-19,共5页农业生物技术（英文版）

基　　金：Supported by Henan Provincial Department of Education (21B350001);Zhengzhou Science and Technology Department (ZZSZX202109;ZZSZX202108)。

摘　　要：Total flavonoids extracted from large-leaved Kuding tea(Ilex latifolia Thunb.) were investigated to develop anti-UV sun-screening agents. The results showed that total flavonoids of I. latifolia Thunb.(TFILT) showed maximum absorb peak at 325 nm between UVB and UVA by wavelength scanning spectrum. TFILT could inhibit the decrease of cell viability induced by UVB irradiation by CCK-8 method, and 0.25 mg/ml of TFILT showed the most significant protective activity on cells. The dermal thickness and collagen fiber density as skin structural characteristic were analyzed by Histological staining. The results showed that TFILT protected mice skin by significantly attenuating the dermal thickness and inhibiting collagen fiber degradation. The mechanism in vivo needs to be further confirmed in future.

关 键 词：Large-leaved Kuding tea Ilex latifolia Thunb. Total flavonoids ANTI-UV 

分 类 号：TQ658.24[化学工程—精细化工]