Quantitative Determination of Sphingolipids by HPLC-ESI/MSn  

作　　者：Jin XIE Rong SONG Li ZHOU Siwen PENG Yanning HUANG Xiaoqi ZHU 

机构地区：[1]Institute of Agricultural and Biological Resources Utilization,Hunan Academy of Agricultural Sciences,Changsha 430100,China

出　　处：《Agricultural Biotechnology》2022年第5期134-135,139,共3页农业生物技术（英文版）

基　　金：Supported by Science and Technology Innovation Project of Hunan Academy of Agricultural Sciences (2021CX32)。

摘　　要：The most predominant sphingoilipids bases are phytosphingosine(phyto-sph) and phytosphingosine-1-phosphate(phyto-S1 P), followed by dihydrosphingosine(dh-sph) and dihydroshingosine-1-phosphate(dh-S1 P). In this study, a fast, sensitive and selective method for determining those four of sphingolipids by high performance liquid chromatography coupled to electrospray ionization ion trap mass spectrometry was introduced. Comparing two methods, method B’s recovery was better than method A. And the limit of detection(LOD) was between 0.003 to 0.013 μM for 20 μl injection.

关 键 词：Sphingoilipids Liquid chromatography Mass spectrometry 

分 类 号：O657.63[理学—分析化学] Q94-33[理学—化学]