荚膜甲基球菌蛋白替代鱼粉对珍珠龙胆石斑鱼生长性能、血清生化指标和肠道健康的影响  

Effects of methanotroph(Methylococcus capsulatus)bacteria meal replacement of fish meal on growth performance,serum biochemical indices and intestinal health of hybrid grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂)

作　　者：梁伟兴 王嘉 谭北平 迟淑艳 LIANG Weixing;WANG Jia;TAN Beiping;CHI Shuyan(Aquatic Economic Animal Nutrition and Feed Laboratory,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Calysta(Shanghai)Business Information Consulting Co.,Ltd,Shanghai 200041,China)

机构地区：[1]广东海洋大学水产学院,水产动物营养与饲料实验室,广东湛江524088 [2]恺勒司(上海)商务信息咨询有限公司,上海200041

出　　处：《水产学报》2022年第10期1969-1979,共11页Journal of Fisheries of China

基　　金：国家重点研发计划(2019YFD0900200);广东省普通高校重点领域专项-服务乡村振兴重点领域专项(2020ZDZX1034);湛江市科技计划项目(2020A02001)。

摘　　要：为了研究荚膜甲基球菌蛋白(MBM)替代鱼粉对珍珠龙胆石斑鱼生长性能和肠道健康的影响,实验以鱼粉含量40%为对照组(MBM0),在饲料中添加2%、4%、8%和12%的荚膜甲基球菌蛋白(MBM),分别替代饲料中鱼粉含量的5%(MBM5)、10%(MBM10)、20%(MBM20)和30%(MBM30),配制5组等氮等脂饲料,投喂初始体质量为(28.85±0.04)g的石斑鱼8周。结果显示,各组石斑鱼存活率均为100%;各替代组增重率、特定生长率、摄食率、饲料系数和蛋白质效率与对照组相比无显著差异;各替代组血清甘油三酯、总胆固醇含量、低密度脂蛋白含量和谷草转氨酶活性显著低于对照组,各组间血清白蛋白和高密度脂蛋白含量无显著性差异;各替代组肠道总抗氧化能力和超氧化物歧化酶活性高于对照组,丙二醛含量低于对照组,但均无显著性差异;各组肠道肌层厚度无显著差异,MBM10、MBM20和MBM30组肠道绒毛高度和宽度与对照组相比无显著差异,MBM10、MBM20、MBM30组和对照组肠道绒毛高度显著高于MBM5组,MBM30组肠道绒毛宽度显著高于MBM5组。实验表明,当基础饲料中鱼粉含量为40%时,MBM可替代30%的鱼粉,对珍珠龙胆石斑鱼的存活率、生长性能和饲料利用率不产生负面影响,并对降低石斑鱼血脂水平和提高肠道抗氧化能力有一定的促进作用。本研究证实了荚膜甲基球菌蛋白在珍珠龙胆石斑鱼饲料中使用的可行性,对节省石斑鱼饲料鱼粉的使用,降低养殖成本具有推动作用。Methanotroph(Methylococcus capsulatus)bacteria meal(MBM),which has the advantages of high protein content and balanced amino acid composition,is a kind of the single-cell protein produced by M.capsulatus,which using methane as the sole carbon and energy source.In order to investigate the effects of substituting MBM replacement for fish meal on growth performance and intestinal health of hybrid grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂),five groups of isonitrogenous and isolipid diets were prepared by adding 2%,4%,8%and 12%MBM to replace 5%(MBM5),10%(MBM10),20%(MBM20)and 30%(MBM30)fish meal based on control diet,respectively,and hybrid grouper[initial weight(28.85±0.04)g]was fed for 8 weeks.The results showed that the survival rate of grouper in each group was 100%.There were no significant differences in weight gain rate,specific growth rate,feed intake rate,feed conversion ratio and protein efficiency ratio among replacement groups compared with control group(P>0.05).In whole fish composition,the moisture in all replacement groups and the ether extract in MBM20 and MBM30 were significantly higher than those in control group(P<0.05),but the crude protein of whole fish and the moisture,ether extract,crude protein,total essential amino acids and flavor amino acids of muscle showed no significant difference in all groups(P>0.05).Serum triglyceride,total cholesterol,low density lipoprotein and glutamic-oxalacetic transaminase in replacement groups were significantly lower than those in control group(P<0.05),but there were no significant differences in serum albumin and high density lipoprotein among all groups(P>0.05).Intestinal total antioxidant capacity and superoxide dismutase activity in replacement groups were higher than those in control group,while malondialdehyde content was lower than that in control group,but there were no significant differences(P>0.05).There was no significant difference in the thickness of intestinal muscle layer among all groups(P>0.05),and no significant difference in the

关 键 词：珍珠龙胆石斑鱼 荚膜甲基球菌蛋白 鱼粉替代 生长性能 肠道健康 

分 类 号：S963.7[农业科学—水产养殖]