Arc weakens synapses by dispersing AMPA receptors from postsynaptic density via modulating PSD phase separation  

作　　者：Xudong Chen Bowen Jia Yoichi Araki Bian Liu Fei Ye Richard Huganir Mingjie Zhang 

机构地区：[1]Division of Life Science,Hong Kong University of Science and Technology,Clear Water Bay,Kowloon,Hong Kong,China [2]Department of Neuroscience and Kavli Neuroscience Discovery Institute,Johns Hopkins University School of Medicine,Baltimore,MD,USA [3]School of Life Sciences,Southern University of Science and Technology,Shenzhen,Guangdong,China

出　　处：《Cell Research》2022年第10期914-930,共17页细胞研究（英文版）

基　　金：supported by a grant from the Minister of Science and Technology of China(2019YFA0508402);a grant from the National Science Foundation of China(82188101);grants fromResearchGrantCouncilof HongKong(AoE-M09-12,16104518 and16101419);a HFSP Research Grant(RGP0020/2019)to M.Z.and_a grant from National Institute of Health(RO1 NS036715)to R.H.

摘　　要：In response to stimuli,the immediate early gene product Arc can acutely down-regulate synaptic strength by removing AMPA receptors(AMPARs)from synapses and thus regulate synaptic plasticity.How Arc,a scaffold protein,can specifically facilitate synaptic removal of AMPARs is unknown.We found that Arc directly antagonizes with PSD-95 in binding to TARPs,which are the auxiliary subunits of AMPARs.Arc,in a highly concentration-sensitive manner,acutely disperses TARPs from the postsynaptic density(PSD)condensate formed via phase separation.TARPs with the Ser residue in the“P-S-Y”-motif of its tail phosphorylated are completely refractory from being dispersed by Arc,suggesting that Arc cannot displace AMPARs from PSDs in active synapses.Conversely,strengthening the interaction between Arc and TARPs enhances Arc’s capacity in weakening synapses.Thus,Arc can specifically and effectively modulate synaptic AMPAR clustering via modulating PSD phase separation.Our study further suggests that activity-dependent,bi-directional modulation of PSD condensate formation/dispersion represents a general regulatory mechanism for synaptic plasticity.

关 键 词：AMPA PHASE removing 

分 类 号：Q2[生物学—细胞生物学]