Retractosomes:small extracellular vesicles generated from broken-off retraction fibers  

作　　者：Yizheng Wang Shuaixin Gao Yuheng Liu Dongju Wang Boqi Liu Dong Jiang Catherine C.L.Wong Yang Chen Li Yu 

机构地区：[1]State Key Laboratory of Membrane Biology,Tsinghua University-Peking University Joint Center for Life Sciences,Beijing Frontier Research Center for Biological Structure,School of Life Sciences,Tsinghua University,Beijing,China [2]Joint Graduate Program of Peking-Tsinghua,National Institute of Biological Sciences,Tsinghua University,Beijing,China [3]Center for Precision Medicine Multi-Omics Research,Peking University Health Science Center,Peking University,Beijing,China [4]School of Basic Medical Sciences,Peking University Health Science Center,Beijing,China [5]Peking-Tsinghua Center for Life Sciences,Beijing,China [6]Peking University First Hospital,Beijing,China [7]Advanced Innovation Center for Human Brain Protection,Capital Medical University,Beijing,China

出　　处：《Cell Research》2022年第10期953-956,共4页细胞研究（英文版）

基　　金：supported by the Ministry of Science and Technology of China(2017YFA0503404);the National Natural Science Foundation of China(92054301,31790401,and 32030023);Beijing Municipal Science&Technology Commission(Z201100005320019).

摘　　要：Dear Editor,Various cell protrusions are known to generate extracellular vesicles.A migratory cell generates retractionfibers(RFs)and migrasomes during migration.After the cell migrates away,large amounts of RFs breakoff from the cell and areleftbehind.The fateof RFs afterdetaching from cells is currently unknown.To investigatethefateof RFs,weobservedTspan4-overexpressing L929 cells with live-cell imaging(Supplementary information,Data S1).We found that during migration,RFs are pulled out at the trailingedgeofcells,andmigrasomes soonformonthetipsor branch points of the RFs.At this stage,the Tspan4 signal is evenly distributed along the RFs(Fig.1a).Eventually,the RFs start to break.Inmostcases,thesectionsof RFfarawayfromthecells break first,which is not surprising,as these sectionsof RFare formed earlier.Interestingly,after the RFsbreak up,the Tspan4 signal appears as a large number of small puncta along the paths which were occupied by the RFs(Fig.1a;Supplementary information,Video S1).

关 键 词：fibers TRAIL evenly 

分 类 号：Q2[生物学—细胞生物学]