基于RAPD分子标记的黑麦草遗传多样性分析  

Genetic Diversity Analysis of Lolium perenne Based on RAPD Molecular Marker

作　　者：苗贵东[1,2,3] 吴小梅 杨慧连 牛秋芳 MIAO Gui-dong;WU Xiao-mei;YANG Hui-lian(Key Laboratory of Research and Development of Ethnic Medicinal Bioresources,Xingyi Normal University for Nationalities,Xingyi,Guizhou 562400;Key Laboratory of Chemical Synthesis and Environmental Pollution Control and Remediation Technology,Xingyi,Guizhou 562400;Biology and Chemistry School,Xingyi Normal University for Nationalities,Xingyi,Guizhou 562400)

机构地区：[1]兴义民族师范学院民族药用生物资源研究与开发重点实验室,贵州兴义562400 [2]贵州省化学合成及环境污染控制和修复技术特色重点实验室,贵州兴义562400 [3]兴义民族师范学院生物与化学学院,贵州兴义562400

出　　处：《安徽农业科学》2022年第22期77-81,85,共6页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技厅基础研究项目(黔科合字LH〔2018〕1166号);兴义民族师范学院科研项目(21XYZJ02);贵州省重点学科(培育)-生态学(ZDXK〔2016〕32);贵州省级大学生创新项目(S202110666091和202110666101)。

摘　　要：通过筛选RAPD标记引物,PCR反应体系优化获得最佳PCR反应条件,并运用筛选的RAPD引物进行群体扩增,筛选出6个效果较好的引物用于24个黑麦草个体组成的群体扩增。共获得52个有效条带,其中,多态性条带49条,占94.23%。24个个体构成的群体被分为5个分支,且各分子间存在一定的遗传距离。By screening the RAPD marker primers,the PCR reaction system was optimized to obtain the best PCR reaction conditions,and the screened RAPD primers were used for population amplification.Six primers with better results were screened for population amplification consisting of 24 individuals of Lolium perenne.A total of 52 effective bands were obtained,of which 49 were polymorphic bands,the percentage of polymorphic bands was 94.23%,and the polymorphism was high.The population composed of 24 individuals is divided into 5 branches,and there was a certain genetic distance between each molecule.

关 键 词：黑麦草 RAPD 遗传多样性 

分 类 号：S543.6[农业科学—作物学]