马铃薯早疫病病原菌的分离鉴定及品种早疫病抗性评价  

Identification of the Pathogens of Potato Early Blight and Evaluation of Potato Varieties Resistance to Early Blight

作　　者：李秀华 梁瑞萍 张仙保 李文霞 王亮明 LI Xiu-hua;LIANG Rui-ping;ZHANG Xian-bao(Academy of Agriculture and Animal Husbandry Sciences in Baotou,Baotou,Inner Mongolia 014013)

机构地区：[1]包头市农牧业科学研究院,内蒙古包头014013

出　　处：《安徽农业科学》2022年第22期86-88,122,共4页Journal of Anhui Agricultural Sciences

基　　金：内蒙古自治区科技计划项目“马铃薯种薯露地繁育提质增效关键技术研究”;内蒙古自治区马铃薯种业技术创新中心项目。

摘　　要：为明确包头地区马铃薯早疫病的病原菌,采用常规组织分离法进行分离纯化,依据病原菌的形态特征和致病性,将分离病原菌初步确定为链格孢属Alternaria。rDNA-ITS序列分析表明,病原菌rDNA-ITS区大小为542 bp,经Blast比对,该菌ITS序列与链格孢属Alternaria同源性100%。同时,对部分马铃薯品种的早疫病抗性进行室内接种鉴定,结果表明,供试12个马铃薯品种中,仅“中薯3号”病情指数为23.44,属中抗品种,其余品种均是早疫病抗性品种。In order to identify the pathogen of potato early blight in Baotou area,routine tissue separation method was used,according to the morphological characteristics and pathogenicity of the pathogen,the isolated pathogen was preliminarily identified as Alternaria.Sequence analysis showed that the rDNA-ITS region of the pathogen was 542 bp.Blast comparison showed that ITS sequence of the pathogen had 100%homology with Alternaria.Meanwhile,the resistance of some potato varieties to early blight was identified by indoor inoculation.The results showed that only the disease index of“Zhongshu 3”was 23.44,which was a moderately resistant variety among the 12 potato varieties,and the other varieties were early blight resistant varieties.

关 键 词：马铃薯早疫病 RDNA-ITS序列分析 接种鉴定 抗性品种 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]