玉屏风加苍耳子散加减方对反复呼吸道感染患儿肠道菌群的影响  

Regulatory effect of Yupingfeng Plus Cang'erzi San Decoction on gut microbiota in children with recurrent respiratory tract infection

作　　者：张园园[1] 钏丹 段晶[1] 邢海晶 吴月滢 熊晶晶[1,3] 李檬 许东云[1] 袁嘉丽 黄永坤[1,3] ZHANG Yuan-yuan;CHUAN Dan;DUAN Jing;XING Hai-jing;WU Yue-ying;XIONG Jing-jing;LI Meng;XU Dong-yun;YUAN Jia-li;HUANG Yong-kun(Pediatrics,the First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650032,China;不详)

机构地区：[1]昆明医科大学第一附属医院儿科,云南昆明650032 [2]云南中医药大学基础医学院,云南昆明650500 [3]云南省检验医学重点实验室,云南昆明650032 [4]云南省中医药学分子生物学重点实验室,云南昆明650500

出　　处：《中国微生态学杂志》2022年第9期1053-1060,1065,共9页Chinese Journal of Microecology

基　　金：云南中医学院-院科技厅联合基金重点项目[2017FF117(-005)]

摘　　要：目的研究玉屏风加苍耳子散加减方(YCD)对反复呼吸道感染(RRTI)患儿肠道菌群的影响。方法 选取2018-2019年我院3~6岁11例RRTI患儿,随机分为YCD治疗组6例,泛福舒(OM-85)治疗对照组5例,分别收取治疗前、治疗2周、治疗4周和治疗3月的粪便,同时选取同年龄段的44例健康儿童作为正常对照组并收取粪便。所有标本进行16S rDNA高通量测序,分析YCD和OM-85治疗RRTI患儿病情变化与肠道菌群变化的相关性并预测菌群功能。结果 YCD治疗RRTI患儿4周至3月的疗效好于OM-85(P<0.05)。RRTI患儿与正常儿童的肠道菌群组成结构有显著差异(P<0.001)。RRTI患儿肠道菌群在治疗前后菌群丰富度和多样性均高于正常儿童(P> 0.05),但治疗后的组间差异均较治疗前减小(P>0.05)。YCD治疗前后的拟杆菌门、放线菌门和拟杆菌属、嗜血杆菌属、梭菌ⅩⅣa属、副拟杆菌属、芽殖菌属的相对丰度变化与YCD疗效规律协同并与功能代谢通路相关(r>0.5,P<0.001)。其中,拟杆菌属、副拟杆菌属和芽殖菌属分别为治疗4周和3月的核心菌属。结论 YCD可以调节RRTI患儿肠道菌群失衡来改善患儿病情,尤其在疾病后期为RRTI患儿的防治提供新思路。Objective To explore the effect of Yupingfeng Plus Cang’erzi San Decoction(YCD) on gut microbiota in children with recurrent respiratory tract infection(RRTI). Methods A total of 11 RRTI children aged 3 to 6 were randomly allocated to receive YCD(n=6) or OM-85(n=5);44 healthy children were selected as normal controls. The gut microbiota of the subjects were examined at pre-teatment, 2 weeks, 4 weeks and 3 months of treatment by using pyrosequencing of 16S r RNA genes. Results YCD had a better therapeutic effect on RRTI than OM-85 at 4 weeks to 3 months of treatment(P<0.05). There were obvious differences between RRTI children and healthy children based on β-diversity analysis(P<0.001). The childen with RRTI presented higher bacterial richness and diversity than healthy children at both pre-and post-treatment(P> 0.05), whereas the differences in microbiota structures and compositions reduced after treatment(P>0.05). YCD was associated with the changes of relative abundance of Bacteroidetes and Actinobacteria at phylum level as well as the potential biomarkers of Bacteroides, Haemophilus, Clostridium Ⅹ Ⅳ a, Parabacteroides and Gemmiger at genus level. The potential biomarkers revealed a significant correlation with metabolic pathway(r>0.5, P<0.001), particularly the core bacteria of Bacteroides, Parabacteroides and Gemmiger at 4 weeks and 3 months of treatment, respectively. Conclusion YCD can improve intestinal microbiota dysbiosis in childen with RRTI to alleviate RRTI, especially in advanced stages, which provides a new way to prevent and treat RRTI in childen.

关 键 词：玉屏风加苍耳子散加减方 反复呼吸道感染 肠道菌群 儿童 

分 类 号：R725.6[医药卫生—儿科]