LSL1 controls cell death and grain production by stabilizing chloroplast in rice  

作　　者：Deyong Ren Wei Xie Qiankun Xu Jiang Hu Li Zhu Guangheng Zhang Dali Zeng Qian Qian 

机构地区：[1]State Key Lab of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China [2]College of Modern Agriculure,Zhejiang A&F University,Hangzhou 31006,China [3]National Nanfan Research Institute,Chinese Academy of Agricultural Sciences,Sanya 572024,China

出　　处：《Science China(Life Sciences)》2022年第11期2148-2161,共14页中国科学（生命科学英文版）

基　　金：the National Natural Science Foundation of China(32188102,32071993,31971872,31861143006,U2004204);the Zhejiang Provincial“Ten Thousand Talent Program”Project(2018R52025,2019R52031);the Hainan Yazhou Bay Seed Laboratory(a project of B21HJ0220);the Key Research and Development Program of Zhejiang Province(2021C02056);the Nanfan Special Project,CAAS(ZDXM06);the Central Public-Interest Scientific Institution Basal Research Fund(CPSIBRF-CNRRI-202111,CPSIBRF-CNRRI202110)。

摘　　要：Lesion mutants can be valuable tools to reveal the interactions between genetic factors and environmental signals and to improve grain production.Here we identified a rice(Oryza sativa)mutant,lesion spotleaf1(lsl1),which produces necrotic leaf lesions throughout its life cycle.LSL1 encodes a protein of unknown function and belongs to a grass-specific clade.The lesion phenotype of the lsl1 mutant was sharply induced by shading,and its detached leaves incubated in 6-benzylamino purine similarly formed lesions in the dark.In addition,the lsl1 mutant exhibited reactive oxygen species accumulation and cell death.The terminal deoxynucleotidyl transferase d UTP nick end-labeling(TUNEL)and comet assays revealed that the lsl1 mutant contained severe DNA damage,resulting in reduced grain yield and quality.RNA sequencing,gene expression,and protein activity analyses indicate that LSL1 is required for chloroplast function.Furthermore,LSL1 interacts with Psa D and PAP10 to form a regulatory module that functions in chlorophyll synthesis and chloroplast development to maintain redox balance.Our results reveal that LSL1 maintains chloroplast structure,redox homeostasis,and DNA stability,and plays important roles in the interaction between genetic factors and environmental signals and in regulating grain size and quality.

关 键 词：lesion formation chloroplast function redox homeostasis grain size and quality LSL1 RICE 

分 类 号：S511[农业科学—作物学]