巴西橡胶树YABBY基因家族鉴定及表达分析  

Identification and Expression Analysis of YABBY Gene Family in Hevea brasiliensis

作　　者：张鸿韬 肖小虎[2] 阳江华[2] 秦云霞[2] 龙翔宇[2] 殷红彦 方永军[2] ZHANG Hongtao;XIAO Xiaohu;YANG Jianghua;QIN Yunxia;LONG Xiangyu;YIN Hongyan;FANG Yongjun(College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China;Institute of Rubber Research,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China)

机构地区：[1]海南大学热带作物学院,海南海口570228 [2]中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2022年第11期2188-2198,共11页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金面上项目(No.31770711);中国热带农业科学院橡胶所基本科研业务费项目(No.1630022020023);国家重点研发计划项目(No.2018YFD1000500)。

摘　　要：YABBY基因家族是一类植物特有的转录因子,在植物叶片和花器官的发育以及非生物胁迫应答中起重要的调控作用。本研究从橡胶树基因组中鉴定得到11个HbYABBY家族成员,并从基因结构、启动子顺式作用元件、染色体定位、系统进化及基因表达等方面进行分析。结果显示11个橡胶树HbYABBY基因分布在9条染色体上,编码蛋白的长度在132~241个氨基酸,分子量在14.75~26.41 kDa,启动子区域含有丰富的光响应元件。该基因家族具有显著的组织表达特异性,叶片和花中高表达,而在胶乳和根中基本不表达。叶片的发育过程中,除HbCRC1上调表达,另外9个HbYABBY基因均显著持续下调表达,表明HbYABBY深度参与橡胶树叶片发育调控。转录组测序和荧光定量检测均发现所有HbYABBY成员受高温诱导持续下调表达,但不受低温胁迫诱导,暗示了HbYABBY在橡胶树的高温胁迫中发挥调控作用。本研究以热带经济林木巴西橡胶树为研究对象,对YABBY转录因子基因家族的理化特征、表达及功能进行了初步分析,为该基因家族功能的深度研究提供了理论依据和参考。YABBY genes family is a plant-specific transcription factor,which plays an important role in the development of plant leaves,floral organs and abiotic stress response,but it has not been reported in rubber tree so far.In this study,eleven HbYABBY genes were identified from the genome of Hevea brasiliensis,and the gene structure,promoter regu-latory elements,chromosome location and collinearity,phylogeny and gene expression were analyzed.The results showed that the HbYABBY family genes were distributed on nine chromosomes.The length of the encoded protein was between 141 and 233 amino acids,and the molecular weight was ranged from 14.75 to 26.41kDa.The promoter regions contained response elements such as stress response,among which the number of light response elements was the largest.Gene transcriptional analysis showed that HbYABBY had significant tissue-specific expression profile,eight genes were only highly expressed in leaves and flowers but not in latex and roots.During the leaf development,except for HbCRC1,the expression of the other 9 HbYABBY were significantly down-regulated,which indicated that HbYABBY were deeply involved in the regulation of leaf development of rubber trees.Furthermore,both transcriptional and RT-qPCR experi-ments showed that HbYABBY genes were continuously down-regulated under high temperature treatment,indicating the regulatory roles in high temperature stress responses of rubber trees.In this paper,YABBY genes were firstly reported in H. brasiliensis, the physical and chemical characteristics, expressions and function of the genes were preliminarily studied, which would provide a solid foundation to investigate the functions and evolution of HbYABBY genes.

关 键 词：巴西橡胶树 YABBY基因家族 转录因子 基因表达分析 

分 类 号：S794.1[农业科学—林木遗传育种]