针刺足三里对脓毒症大鼠小肠微血管重建的影响  

Effect of Acupuncture at Zusanli(ST36)on Small Intestinal Microvascular Remodeling in Septic Rats

作　　者：李灵丰 邱露 周有东 寇丹丹 LI Lingfeng;QIU Lu;ZHOU Youdong;KOU Dandan(Dongguan Peoples Hospital,Dongguan 523059,Guangdong,China;Yichang Central Peoples Hospital,Yichang 443000,Hubei,China)

机构地区：[1]东莞市人民医院,广东东莞523059 [2]宜昌市中心人民医院,湖北宜昌443000

出　　处：《中华中医药学刊》2022年第9期213-216,I0040,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：湖北省微循环学会人才培养专项科研基金[HBWXH2018(1)-3]。

摘　　要：目的研究针刺足三里对脓毒症大鼠肠道上皮细胞的血管内皮生长因子(vascular endothelial growth factor,VEGF)、促血管生成素-1(Angiopoietin-1,Ang-1)、Ang-2、基质金属蛋白酶-2(matrix metalloproteinase,MMP-2)、MMP-9的表达的影响。方法选取200只大鼠作为实验对象,选择10只大鼠作为正常对照组,其余制备脓毒症模型,制备成功后选10只作为假手术组,剩余平均分为模型组、假针刺治疗组、足三里治疗组。分别应用免疫组化方法和免疫荧光结合激光共聚焦技术检测各个血管新生调控因子的时空分布及表达细胞类型;采用蛋白质印迹法(Western blot)和荧光定量PCR技术检测各个血管新生调控因子蛋白和基因表达变化。结果模型组、假针刺与足三里治疗组5-溴脱氧嘧啶核苷(BrdU)、血管性血友病因子(vWF)表达高于对照组与假手术组(P<0.05);且足三里治疗组BrdU、vWF表达高于模型组与假针刺治疗组(P<0.05)。模型组、假针刺与足三里治疗组VEGF、Ang-1、Ang-2、MMP-2、MMP-9 mRNA表达量高于对照组与假手术组(P<0.05);足三里治疗组表达量低于模型组与假针刺治疗组(P<0.05)。模型组、假针刺与足三里治疗组VEGF、Ang-1、Ang-2、MMP-2、MMP-9蛋白表达量高于对照组与假手术组(P<0.05);足三里治疗组蛋白表达量低于模型组与假针刺治疗组(P<0.05)。结论针刺足三里对脓毒症大鼠肠组织具有保护作用,其机制与针刺足三里减轻脓毒症大鼠小肠组织VEGF、Ang-1、Ang-2、MMP-2、MMP-9等因子表达以抑制微血管重建有关。Objective To study the effects of acupuncture at Zusanli(ST36)on expressions of vascular endothelial growth factor(VEGF),angiopoietin-1(Ang-1),angiopoietin-2(Ang-2),matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9)in intestinal epithelial cells of septic rats.Methods A total of 200 rats were selected as the experimental objects,10 rats as the normal control group,and the rest were prepared into sepsis model.After successful preparation,10 rats were selected as the sham operation group.The rest were divided into the model group,the sham acupuncture treatment group and the Zusanli(ST36)treatment group.Immunohistochemical method and immunofluorescence combined with laser confocal technique were used to detect the temporal and spatial distribution of cell types and expressions of angiogenesis regulatory factors.The changes of protein and gene expressions of angiogenesis regulators were detected by Western blot and fluorescence quantitative PCR.Results The mRNA expressions of VEGF,Ang-1,Ang-2,MMP-2 and MMP-9 in the model group,sham acupuncture group and Zusanli(ST36)treatment group were higher than those in the control group and sham operation group(P<0.05).The expression in the Zusanli(ST36)treatment group was lower than that in the model group and sham acupuncture treatment group(P<0.05).The expressions of VEGF,Ang-1,Ang-2,MMP-2 and MMP-9 in the model group,sham acupuncture group and Zusanli(ST36)treatment group were higher than those in the control group and sham operation group(P<0.05).The protein expression in the Zusanli(ST36)treatment group was lower than that in the model group and sham acupuncture treatment group(P<0.05).Conclusion Acupuncture at Zusanli(ST36)has a protective effect on intestinal tissue of septic rats.Its mechanism is related to acupuncture at Zusanli(ST36)reducing the expressions of VEGF,Ang-1,Ang-2,MMP-2 and MMP-9 in small intestine of septic rats and inhibiting microvascular remodeling.

关 键 词：针刺 足三里 脓毒症 微血管重建 血管内皮生长因子 

分 类 号：R245.3[医药卫生—针灸推拿学]