机构地区:[1]青岛市畜牧兽医研究所,山东青岛266100 [2]青岛农业大学动物医学学院,山东青岛266109 [3]青岛瑞尔唯特生物技术有限公司,山东青岛266114 [4]青岛派如环境科技有限公司,山东青岛266200
出 处:《畜牧与饲料科学》2022年第6期30-35,共6页Animal Husbandry and Feed Science
基 金:青岛市科技惠民示范引导专项(21-1-4-ny-10-nsh)。
摘 要:[目的]研究菌酶协同饲喂模式对里岔黑猪断奶仔猪生长性能、血清免疫指标和肠道菌群的影响。[方法]选取80头28日龄体重为(6.72±0.12)kg的健康里岔黑猪断奶仔猪,随机分为对照组(基础日粮)、益生菌组(1‰的益生菌)、酶制剂组(1‰的酶制剂)、菌酶协同组(1‰的益生菌+1‰的酶制剂)。试验期为35 d,预饲期为7 d,正饲期为28 d。计算各组平均日增重、平均日采食量、料重比、腹泻指数,测定血清IgM及IgG含量,通过16S rDNA高通量测序分析肠道菌群组成及相对丰度。[结果]与对照组相比,益生菌组、酶制剂组、菌酶协同组平均日采食量显著(P<0.05)提高;酶制剂组、菌酶协同组平均日增重显著(P<0.05)提高,料重比显著(P<0.05)降低;益生菌组和菌酶协同组腹泻指数极显著(P<0.01)降低。益生菌组、酶制剂组、菌酶协同组血清IgG、IgM含量显著(P<0.05)高于对照组。益生菌组、酶制剂组、菌酶协同组肠道菌群Chao1指数显著(P<0.05)高于对照组;门水平上,益生菌组、菌酶协同组拟杆菌门相对丰度显著(P<0.05)高于对照组。[结论]菌酶协同饲喂模式可以提高里岔黑猪断奶仔猪平均日采食量和平均日增重,降低腹泻指数,具有提高断奶仔猪免疫力、改善肠道菌群结构的作用。[Objective]This study was conducted to investigate the synergistic effects of feeding bacteria and enzymes on growth performance,serum immune indexes and intestinal microflora of weaned piglets of Licha black pig.[Method]A total of 80 healthy 28-day-old weaned piglets of Licha black pig with body weights of(6.72±0.12)kg were randomly divided into the following four groups:a control group fed with basal diet,a probiotic group fed with basal diet supplemented with 1‰of probiotic,an enzyme preparation group fed with basal diet supplemented with 1‰enzyme preparation,and a bacteria and enzymes synergistic group fed with basal diet supplemented with 1‰probiotic and 1‰enzyme preparation.The experiment lasted for 35 d,including a 7 d pre-feeding period and a 28 d formal feeding period.The average daily gain,average daily feed intake,ratio of feed to weight gain and diarrhea index were calculated,and the serum levels of IgM and IgG were determined.The composition and relative abundance of intestinal micoflora were examined by 16S rDNA high-throughput sequencing.[Result]Compared with the control group,the average daily feed intake of the probiotic group,enzyme preparation group,and bacteria and enzymes synergistic group increased significantly(P<0.05);the enzyme preparation group and bacteria and enzymes synergistic group had significantly(P<0.05)increased average daily gain and significantly(P<0.05)decreased ratio of feed to weight gain;the diarrhea index of probiotic group and bacteria and enzymes synergistic group extremely significantly(P<0.01)reduced.The probiotic group,enzyme preparation group,and bacteria and enzymes synergistic group had significantly(P<0.05)higher serum levels of IgM and IgG than control group.Significantly(P<0.05)higher Chao1 index of intestinal microflora was observed in probiotic group,enzyme preparation group,and bacteria and enzymes synergistic group compared with control group.At the phylum level,the relative abundance of Bacteroides in probiotic group as well as bacteria and enzyme
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