不同光照强度胁迫下的多型杜氏藻转录组分析  

Transcriptomic analysis of Dunaliella polymorpha under different light intensity stresses

作　　者：高帆[1,2] 杨鹏 王飞[4] 徐璐[1] 汤丽群 GAO Fan;YANG Peng;WANG Fei;XU Lu;TANG Liqun(School of Life Science,Shanxi University,Taiyuan 030006,China;Academic Affairs Office,Shanxi University,Taiyuan 030006,China;Institute of Biotechnology,Shanxi University,Taiyuan 030006,China;School of Physical Education,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学生命科学学院,太原030006 [2]山西大学教务处,太原030006 [3]山西大学生物技术研究所,太原030006 [4]山西大学体育学院,太原030006

出　　处：《生物学杂志》2022年第6期77-83,共7页Journal of Biology

基　　金：国家自然科学基金项目(31800657);山西省高等学校科技创新项目(2019L0041);山西省科技攻关计划项目(201903D321069);2020年度山西省高等学校教学改革创新项目(J2020009);2020年国家级大学生创新创业训练计划项目(202010108018)。

摘　　要：为研究多型杜氏藻在光照胁迫下的应答机制,筛选差异表达基因,分析其功能及参与的代谢通路,以成熟期的多型杜氏藻为研究对象,分别设置遮光对照组、中光强胁迫组和高光强胁迫组,用形态学、荧光参数测定、高通量测序及生物信息学方法分别对其进行细胞、生化及转录组水平的分析。结果显示,光强的升高有利于藻细胞的形态发育与叶绿素合成,中光胁迫组的光合效率高于高光胁迫组和遮光组。9个RNA测序文库中共获得52853条unigenes,其中coding domain sequence(CDS)29025条,41.59%可被7个生物数据库注释,de novo组装了282条contigs,其中N50为1072 bp,GC百分比52.25%。基于基因的差异表达,中光强胁迫组与高光强胁迫组可聚为一簇,下调基因数量(9385)高于上调基因数量(8046)。组间的共有差异基因GO及KEGG通路分析表明,多型杜氏藻光胁迫应答过程中相关基因功能主要与胞内的光合作用催化活性及天线蛋白合成有关,参与的代谢通路主要与光刺激下的新陈代谢过程有关。研究结果为该藻光合机理的深入研究,光合调控关键基因的克隆与表达、特色资源分子选育及其工业应用奠定基础。In order to research the response mechanism of Dunaliella polymorpha under different light stresses,screen differentially expressed genes,and analyze their functions and metabolic pathways,the shading control group,medium light intensity stress group and high light intensity stress group were set up respectively based on the mature algae strains.The analyses were performed by utilizing morphological,fluorescence parameter determination,high-throughput sequencing and bioinformatics methods at the cellular,biochemical and transcriptomic levels,respectively.A total of 52853 unigenes with 29025 coding domain sequences(CDSs)from the nine RNA-seq libraries were obtained in this alga.41.59%of unigenes were annotated in the 7 bio-database.Moreover,a total of 282 contigs were de novo assembled with a 1072 bp N50 and a GC percentage of 52.25%.Furthermore,based on the differentially expressed genes(DEGs),the medium light intensity stress group and high light intensity stress group could be clusted together and the number of down-regulated DEGs(9385)was more than that of the up-regulated ones(8046).The GO and KEGG pathway analyses of the intergroup common DEGs showed that the functions of key genes involved in the light stress response were mainly related to the intracellular photosynthetic catalytic activity and antenna protein synthesis.The main metabolic pathways were related to the metabolic process stimulated by the light intensity.The results laid a foundation for further researching on this algal photosynthetic mechanism,cloning and expression of key photosynthetic regulation genes,molecular breeding and their industrial application in future.

关 键 词：多型杜氏藻 转录组测序 光照强度 差异表达基因 

分 类 号：Q943[生物学—植物学]