附睾4-5段CD147缺失对精子成熟的作用研究  

Effects of CD147 deletion in segments 4-5 of epididymis on sperm maturation

作　　者：田家玮 戴鹏远 陈浩 Jiawei Tian;Pengyuan Dai;Hao Chen(Institute of Reproductive Medicine,Nantong University Medical School,Nantong 226001,China)

机构地区：[1]南通大学医学院生殖医学研究院,南通226001

出　　处：《中华生殖与避孕杂志》2022年第10期995-1002,共8页Chinese Journal of Reproduction and Contraception

基　　金：国家重点研发计划子课题(2018YFC1003602)。

摘　　要：目的研究小鼠附睾上皮表达的CD147对精子成熟的影响。方法利用小鼠附睾4-5段主细胞特异表达的Lcn5-Cre小鼠和CD147^(flox/flox)繁育,获得24只CD147在附睾4-5段特异敲除的小鼠(CD147 cKO-Lcn5)和21只CD147^(flox/flox)对照小鼠。通过计算机辅助精子活力检测(computer-aided sperm analysis,CASA)、A23187诱导精子的顶体反应以及体外受精(invitro fertilization,IVF)等方法评估CD147对小鼠精子功能的影响。结果小鼠附睾头部4-5段敲除CD147后,精子活力和顶体反应的差异均无统计学意义(均P>0.05)。IVF实验结果显示,CD147 cKO-Lcn5小鼠2-细胞率(74.03%±2.93%)相较于CD147^(flox/flox)小鼠(90.59%±2.39%)显著降低(P=0.012)。结论附睾头部4-5段敲除CD147后对精子活力和顶体反应无显著影响,IVF后2-细胞率显著被抑制。Objective To investigate the potential role of CD147 expressed in the mouse epididymal epithelial cells on sperm maturation.Methods Lcn5-Cre mice which were specifically expressed in the principal cells in the segments 4-5 of mouse epididymis were bred with CD147^(flox/flox) to obtain 24 of the CD147 conditional knockout mice(CD147 cKO-Lcn5)and its counterpart CD147^(flox/flox) control mice.Computer-aided sperm analysis(CASA),A23187-induced sperm acrosome reaction,and in vitro fertilization(IVF)were used to evaluate the effects of CD147 on sperm functions.Results After CD147 knockout in the mice segment 4-5 of the caput epididymis,there were no statistically significant differences in sperm motility and acrosome reaction.Of note,the two-cell ratio of CD147 cKO-Lcn5 mice(74.03%±2.39%)was significantly reduced compared with that of CD147^(flox/flox) mice(90.59%±2.39%)in the experiment for IVF(P=0.012).Conclusion CD147 deleted in the 4-5 caput epididymis has limited regulatory effects on sperm motility and acrosome reaction processes,but significantly affected the ratio of two-cell after IVF.

关 键 词：精子成熟 受精 体外 CD147 附睾4-5区段 

分 类 号：R698.2[医药卫生—泌尿科学]