红树老鼠簕来源真菌Talaromyces flavus TGGP35次级代谢产物及其抗氧化活性  

Secondary Metabolites and Antioxidant Activity of a Mangrove-derived Fungus Talaromyces flavus TGGP35 Derived from Acanthus ilicifolius L.

作　　者：蔡瑾 罗运展 刘婧 张学龙 肖媚 韦方芳 张子怡 郑彩娟[1,2] CAI Jin;LUO Yunzhan;LIU Jing;ZHANG Xuelong;XIAO Mei;WEI Fangfang;ZHANG Ziyi;ZHENG Caijuan(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China;Key Laboratory of Hainan Provincial Tropical Medicinal Phytochemistry,Hainan Normal University,Haikou 571158,China)

机构地区：[1]海南师范大学热带药用资源化学教育部重点实验室,化学与化工学院,海南海口571158 [2]海南师范大学海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2022年第3期245-249,共5页Journal of Hainan Normal University（Natural Science）

基　　金：海南省重点研发计划项目(ZDYF2021SHFZ270);海南省自然科学基金项目(220RC593);海南省重大科技计划项目(ZDKJ202008);国家自然科学基金项目(32160108);海南省院士创新平台科研专项资金项目(YSPTZX202030)。

摘　　要：为从Talaromyces属中分离获得更多生物活性显著的次级代谢产物,本文对一株药用红树老鼠簕(Acanthus ilicifolius L.)来源真菌Talaromyces flavus TGGP35的次级代谢产物及其抗氧化活性进行了研究。综合运用正相硅胶柱层析、Sephadex LH-20凝胶柱层析、半制备HPLC、薄层色谱TLC等色谱分离方法,对该菌发酵产物乙酸乙酯粗浸膏部位进行系统分离纯化;综合利用1DNMR、MS等波谱方法鉴定化合物的结构;采用抗氧化活性模型对化合物进行生物活性评价。从内生真菌T. flavus TGGP35中分离得到7个单体化合物,分别为physcion(1),paeciloxanthone(2),5-[(3E,5E)-3,5-nonadienyl]-1,3-benzenediol(3),trans-ferulic acid(4),3,4-二甲氧基甲苯(5),对羟基苯甲醛(6)和对羟基苯乙酮(7)。从Talaromyces属真菌中分离鉴定了7个次级代谢产物,抗氧化活性测试结果表明,化合物6具有显著的抗氧化活性,其IC50值为0.12 mmol/L,强于阳性对照trolox(IC_(50)值为0.23 mmol/L)。In order to obtain more secondary metabolites with significant biological activity from the genus Talaromyces,the secondary metabolites and antioxidant activities of a medicinal mangrove-derived fungus Talaromyces flavus TGGP35derived from Acanthus ilicifolius L. was investigated. Normal phase silica gel column chromatography, Sephadex LH-20 gel column chromatography, semi-preparative HPLC, thin layer chromatography TLC and other chromatographic separation methods were used to isolate secondary metsbolites from the crude ethyl acetate extract of the fermentation product of the fungus. The structures of the compounds were identified by 1DNMR, MS and other spectral methods. The antioxidant activity model was used to evaluate the biological activity of the compounds. Seven known compounds were isolated from the endophytic fungus T. flavus TGGP35, including physcion(1), paeciloxanthone(2), 5-[(3E,5E)-3,5-nonadienyl]-1,3-benzenediol(3), trans-ferulic acid(4), 3,4-dimethoxytoluene(5), p-hydroxybenzaldehyde(6), p-hydroxyacetophenone(7). Compound 6 had strong antioxidant activity, with the IC50values of 0.12 mmol/L, which was stronger than the positive control trolox with the IC50values of 0.23 mmol/L.

关 键 词：老鼠簕 Talaromyces flavus 次级代谢产物 抗氧化活性 

分 类 号：R281.4[医药卫生—中药学]