基于UHPLC-Q-Exactive Orbitrap MS技术的异绿原酸A大鼠体内代谢产物鉴定  

Identification of metabolites of isochlorogenic acid A in rats based on UHPLC-Q-Exactive Orbitrap MS

作　　者：邓志鹏 张萧 田海涛 DENG Zhi-peng;ZHANG Xiao;TIAN Hai-tao(School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《山东科学》2022年第6期58-64,共7页Shandong Science

基　　金：山东省自然科学基金面上项目(ZR2019MH051)。

摘　　要：为了解异绿原酸A在大鼠体内的代谢情况,对大鼠经灌胃给予异绿原酸A后粪便、尿液、血浆中的代谢产物进行鉴定。按20 mg/kg的剂量灌胃给予大鼠异绿原酸A,收集给药后不同时间点的粪便、尿液、血浆样品,经处理后采用UHPLC-Q-Exactive Orbitrap MS技术对样品进行分析。根据分析得到的化合物相对保留时间、质荷比、特征碎片离子以及相关文献报道,从大鼠粪便、尿液、血浆中共鉴定出包括异绿原酸A原形成分在内的10个成分,其中粪便中4个,尿液中6个。其代谢途径主要为氢化、甲基化、葡萄糖醛酸化、硫酸化等。本实验确证了异绿原酸A在大鼠体内的代谢产物,从而明确其药效物质基础,同时为异绿原酸A进一步研发提供实验依据。Rats were intragastrically administered with isochlorogenic acid A to identify the metabolites in their feces, urine, and plasma. Rat feces, urine, and plasma samples were collected at different time points after intragastric administration of isochlorogenic acid A at a dose of 20 mg/kg. The treated samples were analyzed via UHPLC-Q-Exactive Orbitrap MS. According to the relative retention time, mass/charge ratio, and characteristic fragment ions, 10 metabolites including the parent compound were identified. Among them, four metabolites from feces and six from urine samples were obtained. the main metabolic pathways included hydrogenation, methylation, glucuronidation, and sulfatation. The study identified the metabolites of isochlorogenic acid A in rats, clarified the pharmacodynamic material basis of isochlorogenic acid A, and provided the material basis for further research and development.

关 键 词：异绿原酸A Orbitrap MS技术 代谢产物 代谢途径 灌胃给药 

分 类 号：R285.5[医药卫生—中药学]