梅毒感染患者血清抗核抗体、抗心磷脂抗体检测及其在临床诊断中的意义  

Detection and Clinical Significance of Serum Antinuclear Antibody and Anticardiolipin Antibody Detection in Patients with Syphilis Infection

作　　者：柯秀霞 张晓琍[2] KE Xiu-xia;Zhang Xiao-li(Department of Laboratory Medicine,Gutian County Hospital,Ningde 352264,China;Department of Laboratory Medicine,Union Hospital Affiliated to Fujian Medical University,Fuzhou 350001,China)

机构地区：[1]福建省古田县医院检验科,古田352200 [2]福建医科大学附属协和医院检验科,福州350001

出　　处：《四川解剖学杂志》2022年第3期74-77,共4页Sichuan Journal of Anatomy

摘　　要：目的:探究梅毒感染患者血清抗核抗体(ANA)、抗心磷脂抗体(ACA)抗体检测及其在临床诊断中的意义.方法:选择2019年1月至2021年10月期间收集的210例性病患者为研究对象,共获取210份血液标本,均行梅毒螺旋体颗粒凝胶试验(TPPA)和化学发光法(CLIA)检测梅毒螺旋体(TP),同时测定血清ANA、ACA.以TPPA测定结果为"金标准",将CLIA检测结果阳性的血液标本再分为ANA阳性组、ACA阳性组、ANA与ACA双阳性组、ANA与ACA双阴性组,分析这4组CLIA检测结果与TPPA检测结果的不符合率.结果:检测结果显示,210例患者血液标本TPPA检测阳性126例、CLIA检测阳性119例,CLIA检测诊断梅毒感染的特异度、阳性预测值分别为90.48%、93.28%;ANA阳性组、ACA阳性组、ANA与ACA双阳性组的CLIA检测结果与TPPA检测结果的不符合率分别为3.70%、6.06%、4.76%,ANA与ACA双阴性组与TPPA检测比较,差异无统计学意义(P>0.05).结论:ANA、ACA检测指标与CLIA+TPPA联合检测,可改善临床上对梅毒感染患者的鉴别水平,可作为梅毒的辅助检测指标,以提高整体诊断的精确度.Objective:To investigate the detection and clinical significance of serum antinuclear antibody(ANA)and anticardiolipin antibody(ACA)in patients with syphilis infection.Methods:A total of 210 patients with sexually transmitted disease(STD)collected from January 2019 to October 2021 were selected as the research subjects.A total of 210 blood samples were obtained for detecting.Treponema pallidum test(TPPA)and the chemiluminescence method(CLIA)was used to detect treponema pallidum(TP),and the serum ANA(immunofluorescence method)and ACA(ELISA method)were measured further with the same sampleds.The TPPA measurement result was used as the"gold standard"to compare the CLIA and TPPA detection results.And the positive CLIA detection results were divided further into the ANA positive group,the ACA positive group,the ANA and ACA double positive group,and the ANA and ACA double negative group for analyzing the non-conformity rate of CLIA and TPPA test results.Results:One hundred and twenty-six cases were positive for TPPA and 119 cases were positive for CLIA among the 210 cases.The specificity and positive predictive value of CLIA for diagnosing syphilis infection were 90.48%and 93.28%,respectively.The non-conformity rates of TPPA and CLIA in the ANA positive group,the ACA positive group and the ANA and ACA double positive group were 3.70%,6.06%,and 4.76%,respectively,and there was no significant diference in comparison with the ANA and ACA double-negative groups(P>0.05).Conclusion:The combined detection of ANA and ACA detection indicators combined with CLIA+TPPA can improve the clinical identification of syphilis-infected patients,and can be used as auxiliary detection indicators to improve the overall diagnostic accuracy.

关 键 词：梅毒 抗核抗体 梅毒螺旋体 抗心磷脂抗体 

分 类 号：R759.1[医药卫生—皮肤病学与性病学] R446.6[医药卫生—临床医学]