H-ras、ERK1和Ki-67在鼠口腔癌变黏膜中的表达  

Expressions of H-ras,ERK1 and Ki-67 in Mucosa of Mouse Oral Cancer

作　　者：谢天会 孟宪瑞 岳阳丽[3] XIE Tianhui;MENG Xianrui;YUE Yangli(Department of Stomatology,Zhengzhou Stomatological Hospital,Zhengzhou 450052,China;Department of Stomatology,the Third People’s Hospital of Puyang,Puyang 457000,China;Department of Stomatology,College of Stomatology of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州市口腔医院口腔科,河南郑州450052 [2]濮阳市第三人民医院口腔科,河南濮阳457000 [3]郑州大学口腔医学院口腔病理教研室,河南郑州450052

出　　处：《河南医学研究》2022年第22期4037-4041,共5页Henan Medical Research

摘　　要：目的检测鼠口腔黏膜癌变组织、增生组织和正常组织中H-ras、ERK1和Ki-67的表达,初步探讨H-ras、ERK1和Ki-67在鼠肿瘤发生发展中的作用。方法应用免疫组化SP法检测鼠口腔正常黏膜(15例)、增生黏膜(26例)和癌变黏膜(28例)中H-ras、ERK1和Ki-67的表达。结果H-ras、ERK1和Ki-67蛋白在鼠口腔正常黏膜中不表达或微表达,在异常增生黏膜和癌黏膜中表达依次增高,3组间差异有统计学意义(P<0.01)。经spearman相关性分析,H-ras、ERK1和Ki-67蛋白染色强度与细胞增殖程度相关(P<0.05)。H-ras、ERK1和Ki-67蛋白水平呈正相关(P<0.01)。结论H-ras、ERK1和Ki-67蛋白在鼠口腔癌的发生发展中发挥重要作用。Objective To detect the expression of H-ras,ERK1 and Ki-67 in mouse oral cancer,hyperplastic tissue and normal tissue.To find the role of H-ras,ERK1 and Ki-67 in tumorigenesis and development in mouse oral squamous cell carcinoma.Methods The expressions of H-ras、ERK1 and Ki-67 in mouse oral normal mucosa(15 cases),mucosal hyperplasia(26 cases),cancer mucosa(28 cases)were detected using immunohistochemistry.Results H-ras,ERK1 and Ki-67 protein in oral normal mucosa or no expression in the micro-expression,and mucosal dysplasia in the mucosa of oral cancer were increased,the difference between the three groups was statistical difference(P<0.01).Spearman correlation analysis showed that the staining intensity of H-ras,ERK1 and Ki-67 protein was correlated with the degree of cell proliferation(P<0.05).The protein levels of H-ras,ERK1 and Ki-67 were positively correlated(P<0.01).Conclusion H-ras,ERK1 and Ki-67 may play an important role in the carcinogenesis and development of mouse oral cancer.

关 键 词：口腔癌 鼠 异常增生 H-RAS ERK1 KI-67 

分 类 号：R34[医药卫生—基础医学]