健脾理气方改善功能性消化不良大鼠十二指肠微炎症的作用机制  

Effect of Jianpi Liqi Decoction on the improvement of micro-inflammation in the duodenum of rats with functional dyspepsia

作　　者：常雄飞 沈凌宇 张立宏 谭海成 CHANG Xiong-fei;SHEN Ling-yu;ZHANG Li-hong;TAN Hai-cheng(Department of Gastroenterology,Shunyi Hospital of Traditional Chinese Medicine,Beijing 101300,China)

机构地区：[1]北京中医医院顺义医院脾胃病科,北京101300

出　　处：《北京中医药》2022年第9期978-982,共5页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(82004270);北京市属医院科研培育计划项目(PZ2020024);北京中医医院顺义医院院级课题(SYYJ-201803)。

摘　　要：目的观察健脾理气方改善功能性消化不良(FD)大鼠十二指肠微炎症的作用机制。方法将24只SD雄性大鼠随机分为正常组6只及造模组18只,采用夹尾应激方法建立FD大鼠模型。将造模组随机分为FD模型组、中药治疗组及促肾上腺皮质激素释放激素(CRH)拮抗剂组,正常组及模型组予纯净水灌胃,中药治疗组予健脾理气方水煎剂灌胃7 d,自造模第1天开始至实验结束,拮抗剂组每日8时夹尾应激方法开始前给予大鼠腹腔注射CRH特异性受体拮抗剂。干预结束后取各组十二指肠、下丘脑及血清,HE染色观察十二指肠基本结构形态;使用Western blotting及ELISA法分别测定下丘脑、十二指肠及血清中CRH含量;Western blotting法测定十二指肠组织中P物质(SP)、嗜酸性粒细胞主要碱性蛋白(MBP)、肥大细胞类胰蛋白酶(Tryptase)蛋白表达。结果各组十二指肠结构形态未见明显异常,未见溃疡、肠绒毛破坏及明显炎症等改变。各组下丘脑CRH含量比较,差异无统计学意义(P>0.05);模型组十二指肠、血清CRH含量均高于正常组(P<0.05);中药治疗组十二指肠、血清CRH含量均低于模型组(P<0.05);拮抗剂组血清CRH含量低于模型组(P<0.05),但十二指肠CRH含量与模型组比较差异无统计学意义(P>0.05)。模型组十二指肠SP含量、MBP及Tryptase蛋白表达均高于正常组(P<0.05);中药治疗组十二指肠SP含量、MBP及Tryptase蛋白表达均低于模型组(P<0.05);拮抗剂组十二指肠Tryptase蛋白表达低于模型组(P<0.05),SP含量、MBP蛋白表达低于模型组,但差异无统计学意义(P>0.05)。结论健脾理气方可能通过抑制应激状态下以SP为介导的脑肠轴激活,从而改善十二指肠嗜酸性粒细胞、肥大细胞浸润等微炎症,对FD起到治疗作用。Objective To study the effect of Jianpi Liqi Decoction on the improvement of micro-inflammation in duodenum of rats with functional dyspepsia(FD).Methods 24 SD male rats were randomly divided into normal group(6 rats)and model group(18rats).The FD rat model was established by tail-clamping stress.Then the model group was randomly divided into FD model group,Chinese herbal medicine treatment group and CRH antagonist group.The normal and model group were given pure water by intragastric administration,while the Chinese herbal medicine treatment group was given Jianpi Liqi Decoction by intragastric administration,for 7 days and the CRH antagonist was injected intraperitoneally to the antagonist group before tail-clamping stress was used at 8 o’clock every day from the 1st day of modeling till the end of modeling.After the intervention finished,the deudenum,hypothalamus,and serum of the rats in each group were taken,and HE staining was used to observe the basic structure of duedenum,and Western blotting and ELISA to measure the content of Corticotropin-Releasing Hormone(CRH)in the hypothalamus,duedenum and serum,and Western blotting to measure the content of substance P(SP),eosinophilic major basic protein(MBP),and the expression of tryptase protein in mast cells in the duodenal tissues.Results There was no obvious abnormality in the structure and morphology of duodenum,ulcer,intestinal villi destruction and obvious inflammation in each group were not seen.There was no significant difference in the CRH content in the hypothalamus of rats in each group(P>0.05).The content of CRH in duodenum and serum of model group was higher than that of normal group(P<0.05).The content of CRH in duodenum and serum in the treatment group was lower than that in the model group(P<0.05).Serum CRH content in antagonist group was lower than that in model group(P<0.05),but there was no significant difference in duodenal CRH content between antagonist group and model group(P>0.05).SP content,MBP and Tryptase protein expression in duodenum

关 键 词：健脾理气方 功能性消化不良 脑肠轴 十二指肠 微炎症 大鼠 

分 类 号：R285.5[医药卫生—中药学]