河蚌葡聚糖HBP-A对豚鼠膝关节软骨退变的影响  

Effect of anodonta glucan HBP-A on cartilage degeneration of the knee in guinea pig

作　　者：潘富伟 王翔[1] 熊轶喆 张明才[1] 陈博[1] 徐震球 孙蓉[2] 詹红生[1] 杜国庆[1] PAN Fu-wei;WANG Xiang;XIONG Yi-zhe;ZHANG Ming-cai;CHEN Bo;XU Zhen-qiu;SUN Rong;ZHAN Hong-sheng;DU Guo-qing(Institute of Orthopedics,Shang-hai Academy of Traditional Chinese Medicine,Shanghai 201203,China;Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai201203,China)

机构地区：[1]上海市中医药研究院骨伤科研究所,上海201203 [2]上海中医药大学附属曙光医院,上海201203

出　　处：《中国矫形外科杂志》2022年第19期1781-1785,共5页Orthopedic Journal of China

基　　金：国家自然科学基金面上项目(编号:82174403,81774340);上海中医药大学预算内项目(编号:2021LK079);上海市重中之重临床重点学科建设项目“中医骨伤科学”(编号:2017ZZ02024);“工效筋骨学”上海市中医药新兴交叉学科资助计划建设项目(编号:沪卫计中发2017[024]号);上海中医药大学附属曙光医院四明青年基金项目(编号:SGKJ-201913);2021年度上海中医药大学“研究生创新培养专项”项目(编号:Y2021088)。

摘　　要：[目的]观察关节内注射不同剂量河蚌葡聚糖(anodonta glucan,HBP-A)对豚鼠膝关节软骨退变及相关标志物的影响。[方法]豚鼠20只随机分为五组,分别为正常组、模型组、低浓度组、中浓度组和高浓度组,每组4只。正常组行右膝假手术处理,其余动物均行右膝前交叉韧带切断,建立骨性关节炎模型。术后3 d,正常组和模型组右膝关节内注射生理盐水,而低、中、高剂量组分别注射7.5 mg/ml、15 mg/ml、30 mg/ml的HBP-A 200μl,每周注射1次,连续5周。5周后处死动物,取右膝关节标本行组织学、免疫组织化学和qPCR检测。[结果]组织学观察见,与正常组相比,模型组出现显著软骨退变,而注射HBP-A组织结构显著改善。模型组较正常组OARSI评分显著增加(P<0.05),在给予低、中、高浓度HBP-A关节腔注射,OARS评分呈剂量依赖性显著降低(P<0.05)。免疫组化方面,模型组较正常组软骨ALP、Ihh、Runx2和MMP13的OD值显著升高(P<0.05),而低、中、高浓度HBP-A关节注射软骨ALP、Ihh、Runx2和MMP13的OD值呈剂量依赖性显著降低(P<0.05)。qPCR检测表明,模型组较正常组软骨IL-1β、Ihh、Runx2和MMP-13的mRNA的相对表达量均显著增加(P<0.05),在给予低、中、高浓度HBP-A关节腔注射,IL-1β、Ihh、Runx2和MMP-13的mRNA的相对表达量均呈剂量依赖性显著降低(P<0.05)。[结论]关节腔内注射HBP-A能够减少或延缓豚鼠膝关节软骨退变程度。[Objective]To explore the effects of intraarticular injection of anodonta glucan(HBP-A)in different dosages on cartilage de⁃generation of the knee,and related markers in guinea pigs.[Methods]Twenty guinea pigs were randomly divided into five groups,including the normal group,model group,low concentration group,medium concentration group and high concentration group,with 4 animals in each group.Four animals in the normal group were treated with right knee sham operation,while the remaining all animals were treated with ante⁃rior cruciate ligament transection of right knee to establish osteoarthritis(OA)model.Three days later,the normal group and model group were injected with normal saline into right knee joint,while the low,medium and high dose groups were injected with 7.5 mg/ml,15 mg/ml and 30 mg/ml of HBP-A 200μl,respectively,once a week for consecutive 5 weeks.Five weeks later,the animals were sacrificed,and the right knee samples were harvested for histological,immunohistochemical and qPCR assays.[Results]With histological observation,signifi⁃cant changes of cartilage degeneration were found in the model group compared with those in the normal group,whereas which significantly reversed in a dosage-dependent manner in the 3 groups with HBP-A in different dose.OARSI score in the model group was significantly higher than that of the normal group(P<0.05),which significantly decreased in a dose-dependent manner in the 3 HBP-A groups(P<0.05).In terms of immunohistochemistry,the optical density(OD)values of ALP,Ihh,Runx2 and MMP13 in the model group were significantly higher than those of the normal group(P<0.05),whereas which declined significantly in the 3 HBP groups in the dose-dependent manner(P<0.05).As results of qPCR assay,the mRNA relative expression levels of IL-1β,Ihh,Runx2 and MMP-13 in the model group were signif⁃icantly higher than those in the normal group(P<0.05),which also reduced significantly after injection of low,medium and high concentra⁃tions of HBP-A into the joint cavity in the d

关 键 词：骨性关节炎 河蚌葡聚糖 关节内注射 软骨退变 

分 类 号：R318[医药卫生—生物医学工程]