Speed vernalization to accelerate generation advance in winter cereal crops  

作　　者：Jin-Kyung Cha Kathryn O'Connor Samir Alahmad Jong-Hee Lee Eric Dinglasan Hyeonjin Park So-Myeong Lee Dominique Hirsz Soon-Wook Kwon Youngho Kwon Kyeong-Min Kim Jong-Min Ko Lee T.Hickey Dongjin Shin Laura E.Dixon 

机构地区：[1]National Institute of Crop Science,RDA,Miryang 50424,Korea [2]School of Biology,Faculty of Biological Sciences,University of Leeds,Leeds LS29JT,UK [3]Queensland Alliance for Agriculture and Food Innovation,The University of Queensland,St.Lucia,QLD,Australia [4]Department of Plant Bioscience,Pusan National University,Miryang 60463,Korea

出　　处：《Molecular Plant》2022年第8期1300-1309,共10页分子植物（英文版）

基　　金：This research was supported by the Research Program for Agricultural Science and Technology Development(project no.PJ01450201)Rural Development Administration;L.T.H.received funding from the Australian Research Council(ARC),project codes DP190102185 and LP170100317;Genotyping of the winter-barley accessions at The University of Queensland was funded through the Grains Research and Development Corporation(GRDC),project code UOQ2005-012RTX;S.A.was supported by a GRDC Postdoctoral Fellowship,project code UOQ1903-007RTX。

摘　　要：There are many challenges facing the development of high-yielding,nutritious crops for future environments.One limiting factor is generation time,which prolongs research and plant breeding timelines.Recent advances in speed breeding protocols have dramatically reduced generation time for many short-day and long-day species by optimizing lightand temperature conditions during plant growth.However,winter crops with a vernalization requirement stillrequire upto 6-10weeks in low-temperature conditions before thetransition to reproductivedevelopment.Here,we tested a suite of environmental conditions and protocols to investigate whether the vernalization process can be accelerated.We identified a vernalization method consisting of exposing seeds at the soil surface to an extended photoperiod of 22 h day:2 h night at 10°C with transfer to speed breeding conditions that dramatically reduces generation time in both winter wheat(Triticum aestivum)and winter barley(Hordeum vulgare).Implementation of the speedvernalization protocolfollowed byspeedbreedingallowed the completion ofuptofivegenerations per yearforwinter wheat or barley,whereas only two generations can be typically completed under standard vernalization and plant growth conditions.The speed vernalization protocol developed in this study has great potential to accelerate biological research and breeding outcomes for winter crops.

关 键 词：speed breeding PHOTOPERIOD temperature wheat BARLEY BREEDING 

分 类 号：S512[农业科学—作物学]