Co-evolved plant and blast fungus ascorbate oxidases orchestrate the redox state of host apoplast to modulate rice immunity  

作　　者：Jiexiong Hu Muxing Liu Ao Zhang Ying Dai Weizhong Chen Fang Chen Wenya Wang Danyu Shen Mary Jeanie Telebanco-Yanoria Bin Ren Haifeng Zhang Huanbin Zhou Bo Zhou Ping Wang Zhengguang Zhang 

机构地区：[1]Department of Plant Pathology,College of Plant Protection,Nanjing Agricultural University,and Key Laboratory of Integrated Management of Crop Diseases and Pests,Ministryof Education,Nanjing,China [2]The Key Laboratory of Plant Immunity,Nanjing Agricultural University,Nanjing,China [3]Genetics and Biotechnology Division,International Rice Research Institute,Los Banos,Laguna 4031,Philippines [4]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China [5]Department of Microbiology,Immunology,and Parasitology,Louisiana State University Health Sciences Center,New Orleans,LA,USA

出　　处：《Molecular Plant》2022年第8期1347-1366,共20页分子植物（英文版）

基　　金：This research was supported by the China National Funds for Innovative Research Groups(31721004);the key program of the Natural Science Foundation of China(NSFC)(32030091);the NSFC Youth Program(31901832);Youth Program for Natural Science Foundation of Jiangsu Province(BK2019054);the program of NSFC-DFG(31861133017);the NSFC program(32172377)。

摘　　要：Apoplastic ascorbate oxidases(AOs)play a critical role in reactive oxygen species(RoS)-mediated innate host immunity by regulating the apoplast redox state.To date,little is known about how apoplastic effectors of the riceblast fungus Magnaportheoryzaemodulate the apoplast redox state of rice to subvert plant immunity.In this study,we demonstrated that M.oryzae MoAo1 is an Ao that plays a role in virulence by modulating the apoplast redox status of rice cells.We showed that MoAo1 inhibits the activity of rice OsAO3and OsAO4,which also regulate the apoplast redox status and plant immunity.In addition,we found that MoAo1,OsAO3,andOsAO4 allexhibit polymorphic variations whosevaried interactions orchestrate pathogen virulence and rice immunity.Taken together,our results reveal a critical role for extracellular redox enzymes during rice blast infection and shed light on the importance of the apoplast redox state anditsregulation inplant-pathogeninteractions.

关 键 词：apoplast redox state RoS ascorbate oxidase POLYMORPHISM rice blast host immunity 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]