雌雄罗氏沼虾应对低氧胁迫的行为生理响应  

Behavioral and physiological responses to hypoxia stress in male and female Macrobrachium rosenbergii

作　　者：邱小龙 江颖琳 蔡雅霜 陈辉 吕晓静 林蠡[1] 李江涛 QIU Xiao-long;JIANG Ying-lin;CAI Ya-shuang;CHEN Hui;LYU Xiao-jing;LIN Li;LI Jiang-tao(Guang-zhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding,Zhongkai University of Agriculture and En-gineering,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院,广州市水产病害与水禽养殖重点实验室,广州510225

出　　处：《应用生态学报》2022年第10期2836-2844,共9页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(32002375);广东省教育厅青年创新人才项目(2020KQNCX029);广东省基础与应用基础研究基金(2020A1515410006)资助。

摘　　要：为查明雌雄罗氏沼虾应对低氧胁迫的行为生理响应,设置6.46(对照)、4.48和3.27 mg·L^(-1)3种溶解氧水平,研究了雌、雄个体在胁迫6 d后肝胰脏和肌肉能量代谢酶活性及游泳和弹跳速度。结果表明:溶解氧从6.46 mg·L^(-1)降至4.48 mg·L^(-1),雌雄个体肌肉有氧代谢酶活性及游泳速度均显著下降,且雄性下降幅度小于雌性,厌氧代谢酶活性并无显著变化;溶解氧继续降至3.27 mg·L^(-1),雌雄个体肌肉有氧代谢酶和厌氧代谢酶活性均显著下降,肝胰脏厌氧代谢酶中的乳酸脱氢酶(LDH)活性及弹跳速度显著下降,且雌性肝胰脏LDH活性下降幅度小于雄性。雌雄罗氏沼虾游泳速度与游泳足肌肉有氧代谢酶活性呈显著正相关,弹跳速度则与腹部肌肉厌氧代谢酶活性呈显著正相关。表明罗氏沼虾可以通过降低能量代谢水平应对低氧胁迫,但这种生理调节会导致运动能力下降,雄性优先将能量分配于肌肉以满足运动,雌性则优先保障肝胰脏能量供应。To explore the physiological and behavioral responses of male and female Macrobrachium rosenbergii un-der hypoxia stress,an experiment with three dissolved oxygen(DO)levels(6.46,4.48 and 3.27 mg·L^(-1),6.46 mg·L^(-1)as control)was conducted.The enzyme activities of energy metabolism in hepatopancreas and muscles of male and female M.rosenbergii were measured after six days of hypoxia stress.The results showed that the enzyme activities of aerobic metabolism in muscles and swimming abilities were significantly decreased as DO decreased from 6.46 mg·L^(-1)to 4.48 mg·L^(-1),with the decreases being less in males than females.There was no significant difference in enzyme activities of anaerobic metabolism.When DO was further decreased to 3.27 mg·L^(-1),the enzyme activities of aerobic metabolism and anaerobic metabolism in muscles significantly decreased.The activity of lactate dehydrogenase(LDH)in anaerobic metabolism of hepatopancreas and tail-flipping speeds significantly de-creased in males and females,with less decrease in females than that in males for LDH activity of hepatopancreas.The swimming ability was positively correlated with the enzyme activities of aerobic metabolism in pleopods muscles.There was significant correlation between tail-flipping abilities and enzyme activities of anaerobic metabolism.M.rosenbergii could reduce its reliance on energy metabolism during hypoxia stress,but with negative consequences on locomotor abilities.The muscles were preferentially powered to meet energy requirements of locomotion in males,while females gave priority to energy supply for hepatopancreas under insufficient oxygen conditions.

关 键 词：罗氏沼虾 溶解氧 能量代谢 游泳 弹跳 

分 类 号：S917.4[农业科学—水产科学]