葡甘露聚糖凝胶通过调节MKP-1表达对宫颈炎大鼠的治疗作用及机制研究  

Therapeutic effect and mechanism of glucomannan gel on cervicitis rats by regulating expression of MKP-1

作　　者：高乔 张润涛 刘聪[1] 李佳 节笑笑 倪艳[1] 康永[1] 杨黎彬 韩文霞 李伟泽 GAO Qiao;ZHANG Run-tao;LIU Cong(Shanxi Institute of Traditional Chinese Medicine,Taiyuan 030012,China;不详)

机构地区：[1]山西省中医药研究院,太原030012 [2]江苏微纳医药科技有限公司

出　　处：《山西中医》2022年第12期54-58,共5页Shanxi Journal of Traditional Chinese Medicine

基　　金：陕西高校青年创新团队建设项目(编号:陕教[2019]90号);陕西省中医药管理局科研课题(编号:2019-ZZ-ZY010);陕西省科技厅面上项目(编号:2020JM-610);国家自然科学基金项目(编号:82004075);江苏省扬中市第十批“江雁计划”人才项目(编号:扬人才办[2021]26号);山西省“四个一批”科技兴医创新计划项目(编号:2020SYS09)。

摘　　要：目的:探讨葡甘露聚糖凝胶治疗宫颈炎大鼠的疗效及作用机制。方法:选取60只雌性SD大鼠采用苯酚胶浆进行阴道内注射建立宫颈炎模型,另取10只作为空白对照组;造模后将大鼠分为模型对照组、葡甘露聚糖凝胶高、中、低剂量组(1.2 g·kg^(-1)、0.6 g·kg^(-1)、0.3 g·kg^(-1))、复方甲硝唑阴道栓组(0.4 g·kg^(-1))、凝胶基质组各10只,采用阴道内灌注给药。治疗1周结束后,观察各组大鼠TNF-α、IL-1β、IL-6、IL-8、EGFR水平及宫颈组织病理形态学变化;采用RT-PCR和Western blot法检测大鼠宫颈组织中MKP-1 mRNA及蛋白的表达。结果:与空白组比较,模型组大鼠血清中TNF-α、IL-1β、IL-6、IL-8的含量均明显升高,EGFR含量均明显降低(P﹤0.01);与模型组比较,葡甘露聚糖凝胶各剂量组和复方甲硝唑组大鼠血清中的TNF-α、IL-1β、IL-6、IL-8的含量有不同程度的下降,EGFR含量有不同程度的升高(P﹤0.01或P﹤0.05)。宫颈组织病理结果显示,模型组宫颈被覆鳞状上皮,上皮可见少量中性粒细胞浸润,可见较多炎性细胞散在浸润,与空白组比较,炎症反应轻度加重,葡甘露聚糖凝胶给药组宫颈被覆鳞状上皮,固有层胶原丰富,排列紧密,可见少量炎性细胞散在浸润,炎症反应减轻。与空白组及模型组比较,葡甘露聚糖凝胶高、中剂量组与复方甲硝唑组能够明显升高MKP-1的mRNA和蛋白水平(P﹤0.01)。结论:葡甘露聚糖凝胶对宫颈炎大鼠有良好的治疗效果,调节MKP-1表达可能是其发挥抗炎效果的作用机制。Objective:To approach the efficacy and mechanism of glucomannan gel on cervicitis rats.Methods:60 female SD rats were selected and established into the model of cervicitis by intravaginal injection of phenol mucilage,others 10 rats were selected as blank control group.After modeling,the rats were divided into model control group,high-,medium-and low-dose glucomannan gel groups(1.2 g·kg^(-1),0.6 g·kg^(-1),0.3 g·kg^(-1)),compound metronidazole vaginal suppositories group(0.4 g·kg^(-1))and gel matrix group,10 rats in each group,and the rats were administered by intravaginal infusion.After treatment for one weak,the levels of TNF-α,IL-1β,IL-6,IL-8 and EGFR as well as the change of pathomorphology of cervical tissue in rats were observed in each group.mRNA and protein expression of MKP-1 in cervical tissues of rats were detected by RT-PCR and Western blot.Results:Compared with blank group,the contents of TNF-α,IL-1β,IL-6 and IL-8 in serum of rats in model group were significantly increased,while the content of EGFR was significantly decreased(P﹤0.01).Compared with model group,the contents of TNF-α,IL-1β,IL-6 and IL-8 in serum of rats in each dose group of glucomannan gel and in compound metronidazole group were decreased in different degrees,while the content of EGFR was increased in different degrees(P﹤0.01 or P﹤0.05).According to the pathological results of cervical tissue,in the model group,the cervix was covered with squamous epithelium,a small amount of neutrophil infiltration was visible on the epithelium;the scattered infiltration of more inflammatory cells was visible,and compared with the blank group,the inflammatory reaction was slightly aggravated.In the glucomannan gel administration group,the cervix was covered with squamous epithelium,the lamina propria collagen was rich and tightly arranged;the scattered infiltration of a small amount of inflammatory cells was visible,and the inflammatory reaction was reduced.Compared with blank group and model group,high-and mediumdose glucomannan gel g

关 键 词：宫颈炎 葡甘露聚糖凝胶 丝裂原活化蛋白激酶磷酸酶1 实验研究 

分 类 号：R256.11[医药卫生—中医内科学]