梨品种‘玉露香’抗火疫病蛋白质组学分析  

Proteomics analysis on the resistance of pear cultivar ‘Yuluxiang’ to fire blight disease

作　　者：吴瑞瑞 叶云锋 陈松余 徐幼平 楼兵干[2] 蔡新忠[1,2] WU Ruirui;YE Yunfeng;CHEN Songyu;XU Youping;LOU Binggan;CAI Xinzhong(Hainan Institute of Zhejiang University,Sanya 572025,China;Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang Provincial Key Laboratory of Biology of Crop Pathogen and Insects,Zhejiang University,Hangzhou 310058,China;Centre of Analysis and Measurement,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学海南研究院,三亚572025 [2]浙江大学农业与生物技术学院生物技术研究所,浙江省作物病虫生物学重点实验室,杭州310058 [3]浙江大学分析测试中心,杭州310058

出　　处：《植物保护》2022年第6期90-97,104,共9页Plant Protection

基　　金：国家重点研发计划(2021YFD1400200);新疆维吾尔自治区科技计划(xjlky20190620)。

摘　　要：由解淀粉欧文氏菌Erwinia amylovora引起的火疫病是一种重要的经济病害,严重影响着全球苹果和梨的生产。E.amylovora被列为我国重要的检疫性有害生物。然而,植物抗火疫病分子机制尚不够明确。本研究鉴定了梨品种‘玉露香’对E.amylovora的抗性,开展了抗性相关蛋白质组学分析。嫩枝穿刺接种分析结果表明,‘玉露香’对E.amylovora表现出明显早期抗性。采用高通量蛋白组学分析技术,鉴定获得‘玉露香’和感病品种‘库尔勒香梨’的差异表达蛋白192个,其中包括29个抗病和防卫相关蛋白,13个氧化还原相关蛋白以及1个铜离子转运ATP酶蛋白HMA5。抑菌动态分析结果显示,2 mmol/L Cu^(2+)完全抑制E.amylovora的生长。本研究为进一步揭示梨火疫病抗性分子机制提供了候选基因。Fire blight, caused by the bacterial phytopathogen Erwinia amylovora, is an economically important disease that affects apple and pear production worldwide. E.amylovora has been listed as an important quarantine pest in China. Nevertheless, the molecular mechanisms underlying plant resistance to this pathogen remain largely unknown. In this study, the resistance of pear cultivar ‘Yuluxiang’ to E.amylovora was identified and a proteomics analysis of the resistance was conducted. The results of the shoot-pricking inoculation analyses showed that ‘Yuluxiang’ exhibited obvious resistance to E.amylovora at early stage of infection. Through high-throughput proteomics analysis, 192 proteins differentially expressed between ‘Yuluxiang ’and the susceptible cultivar ‘Korla fragrant pear’ were identified, including 29 disease-resistant and defense-related proteins, 13 redox homeostasis related proteins and one copper transporting ATPase HMA5. Further dynamic bacteria-suppressing analysis demonstrated that the growth of E.amylovora was completely inhibited in the media supplied with 2 mmol/L Cu^(2+). This study provided candidate genes for further revealing the molecular mechanisms that underlie plant resistance to E.amylovora.

关 键 词：梨火疫病 解淀粉欧文氏菌 抗病性 蛋白质组学 铜 

分 类 号：S41[农业科学—植物保护] S436.6