Effects of Mo-Rubbing abdomen manipulation on glucose metabolism and inflammatory factors in rats with type 2 diabetes mellitus  

摩腹对2型糖尿病大鼠糖代谢和炎症因子的影响

作　　者：XIE Zhouyu XU Jingsong KONG Xintian WU Yunchuan YANG Yanping 谢舟煜;徐景崧;孔心甜;吴云川;杨燕萍(School of Acupuncture,Moxibustion and Tuina,Nanjing University of Chinese Medicine,Nanjing 210023,China;不详)

机构地区：[1]School of Acupuncture,Moxibustion and Tuina,Nanjing University of Chinese Medicine,Nanjing 210023,China [2]不详

出　　处：《Journal of Acupuncture and Tuina Science》2022年第5期354-362,共9页针灸推拿医学（英文版）

基　　金：国家中医药管理局国家中医临床研究基地业务建设第二批科研专项课题,No.JDZX2015127;江苏省研究生培养创新工程研究生科研与实践创新计划,No.KYCX20_1538;江苏省大学生创新创业训练计划,No.201910315071Y。

摘　　要：Objective To observe the effect of Mo-Rubbing abdomen manipulation on glucose metabolism and inflammatory factors in rats with type 2 diabetes mellitus(T2DM).Methods Sixty Sprague-Dawley rats were randomly divided into a normal group(n=10)and a group for modeling(n=50)using the random number table method.Rats in the group for modeling were induced to form T2DM models by a high-sugar and high-fat diet combined with intraperitoneal injection of streptozotocin.Thirty successfully modeled rats were randomly divided into a model group,a Mo-Rubbing abdomen group,and a metformin group,with 10 rats in each group.Rats in the normal group and the model group received no intervention,those in the Mo-Rubbing abdomen group received Mo-Rubbing abdomen manipulation,and those in the metformin group received metformin by gavage.After 8-week intervention,fasting insulin(FINS),fasting plasma glucose(FPG),homeostasis model assessment for insulin resistance(HOMA-IR)and area under the curve at the oral glucose tolerance test(AUC-OGTT),as well as serum inflammatory factors interleukin(IL)-1β,IL-6,and tumor necrosis factor(TNF)-αwere detected,and the morphological changes of the pancreas were also observed.Results After the 8-week intervention,the levels of FINS,FPG,HOMA-IR,and AUC-OGTT of rats in the Mo-Rubbing abdomen group were significantly lower than those in the model group(P<0.05);the pancreatic injury degree in the Mo-Rubbing abdomen group and the metformin group was lower than that in the model group.Compared with the model group,the serum IL-1β,IL-6,and TNF-αlevels of rats in the Mo-Rubbing abdomen group were significantly decreased(P<0.05),and the serum IL-1βand TNF-αlevels of the metformin group showed a downward trend;the serum IL-6 and TNF-αlevels in the Mo-Rubbing abdomen group were significantly lower than those in the metformin group(P<0.01).There was a significant positive correlation between FPG with IL-1β,IL-6,and TNF-αin the T2DM rats(P<0.01).Conclusion Mo-Rubbing abdomen manipulation reduces the inflammato目的观察摩腹对2型糖尿病(T2DM)大鼠糖代谢和炎症因子的影响.方法将60只Sprague-Dawley大鼠采用随机数字表法分为正常组(n=10)和造模组(n=50).造模组采用高糖高脂饲料联合腹腔注射链脲佐菌素诱导大鼠形成T2DM模型.将造模成功的30只大鼠再随机分为模型组、摩腹组和二甲双胍组,每组10只.正常组和模型组不干预,摩腹组予以摩腹,二甲双胍组予以二甲双胍灌胃.干预8周后测定各组空腹胰岛素(FINS)、空腹血糖(FPG)、稳态模型的胰岛素抵抗指数(HOMA-IR)和口服葡萄糖耐量试验曲线下面积(AUC-OGTT),检测血清炎症因子白细胞介素(IL)-1β、IL-6和肿瘤坏死因子(TNF)-α,同时观察胰腺形态学变化.结果干预8周后,摩腹组大鼠FINS、FPG、HOMA-IR和AUC-OGTT均显著低于模型组(P<0.05);摩腹组和二甲双胍组胰腺损伤程度均小于模型组.与模型组比较,摩腹组大鼠血清IL-1β、IL-6和TNF-α含量均显著下降(P<0.05),二甲双胍组大鼠血清IL-1β和TNF-α含量均呈下降趋势;摩腹组血清IL-6和TNF-α显著低于二甲双胍组(P<0.01).T2DM大鼠FPG与IL-1β、IL-6和TNF-α均呈显著正相关(P<0.01).结论摩腹能够降低T2DM大鼠炎症反应,改善胰腺的形态学变化,从而达到降低血糖的效果.

关 键 词：TUINA MASSAGE Mo-Rubbing Manipulation Diabetes Mellitus Type 2 Inflammatory Factors Carbohydrate Metabolism 

分 类 号：R2-03[医药卫生—中医学]