参蛤散对H9c2心肌细胞增殖的影响  

Effect of Shen′ge Powder on H9c2 Cardiomyocyte Proliferation

作　　者：邱伯雍 魏易洪[2] 苑素云[2] 毛美娇[2] 曹敏[2] 周端[2] 邓兵[2] 沈琳[2] QIU Boyong;WEI Yihong;YUAN Suyun;MAO Meijiao;CAO Min;ZHOU Duan;DENG Bing;SHEN Lin(First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450000,Henan,China)

机构地区：[1]河南中医药大学第一附属医院,郑州450000 [2]上海中医药大学附属龙华医院,上海200032

出　　处：《中西医结合心脑血管病杂志》2022年第22期4098-4101,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金资助项目(No.81804010);上海中医药大学附属龙华国家中医临床研究基地“龙医育苗”计划(No.LYTD-83);上海中医药大学高水平大学建设经费(No.A1-U1820501030201)。

摘　　要：目的观察参蛤散对H9c2心肌细胞增殖的影响,为参蛤散后续研究提供依据。方法采用H9c2心肌细胞株,制备参蛤散冻干粉进行干预,在24 h、48 h、72 h各时间点,用细胞计数试剂盒-8(CCK-8)法检测细胞吸光度值(OD值),细胞划痕实验检测细胞迁移率。结果24 h参蛤散0.03 mg/mL、0.10 mg/mL、0.30 mg/mL、1.00 mg/mL组OD值高于对照组(P<0.05);48 h参蛤散各浓度组OD值均高于对照组(P<0.05);72 h参蛤散0.01 mg/mL组OD值高于对照组(P<0.05),其余组与对照组比较差异均无统计学意义(P>0.05)。24 h参蛤散0.10 mg/mL、0.30 mg/mL、1.00 mg/mL组细胞迁移率大于对照组(P<0.05);48 h参蛤散0.01 mg/mL、0.10 mg/mL、0.30 mg/mL组细胞迁移率大于对照组(P<0.05);72 h参蛤散0.01 mg/mL、0.30 mg/mL组细胞迁移率大于对照组(P<0.05)。结论参蛤散可以促进H9c2心肌细胞增殖。Objective To explore the effect of Shen′ge Powder(SGP)on H9c2 cardiomyocyte proliferation.Methods The freeze-dried powder of SGP was prepared for intervention of H9c2 cardiomyocyte.Optical density(OD)value was detected by CCK-8 assay,and cell mobility was detected by cell wound healing assay at 24 h,48 h,and 72 h.Results Compared with control group,OD value was increased in SGP 0.03 mg/mL,0.10 mg/mL,0.30 mg/mL,and 1.00 mg/mL groups at 24 h(P<0.05),in all SGP groups at 48 h(P<0.05),and in SGP 0.01 mg/mL group at 72 h(P<0.05).Compared with control group,cell migration rate was increased in SGP 0.10 mg/mL,0.30 mg/mL,and 1.00 mg/mL groups at 24 h(P<0.05),in SGP 0.01 mg/mL,0.10 mg/mL,and 0.30 mg/mL groups at 48 h(P<0.05),and in SGP 0.01 mg/mL and 0.30 mg/mL groups at 72 h(P<0.05).Conclusion SGP can promote H9c2 cardiomyocyte proliferation.

关 键 词：H9C2心肌细胞 增殖 参蛤散 实验研究 

分 类 号：R285[医药卫生—中药学]