Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes  

作　　者：Xinzhu Xu Shu Jia Peng Xi 

机构地区：[1]Department of Biomedical Engineering,College of Future Technology,Peking University,Beijing 100871,China [2]Wallace H.Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University,Atlanta,Georgia 30332,USA [3]UTS-SUStech Joint Research Centre for Bio-medical Materials&Devices,Department of Biomedical Engineering,College of Engineering,Southern University of Science and Technology,Shenzhen,Guangdong 518055,China [4]National Biomedical Imaging Center,Peking University,Beijing 100871,China

出　　处：《Light(Science & Applications)》2022年第10期2271-2275,共5页光（科学与应用)（英文版）

摘　　要：A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice.Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process.Consisting of hollow zero-intensity excitation,single-pixel detection,time-correlated single photon counting,and drift stabilization,the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally.An averaged high-SNR reference,and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation.With performance reaching nearly conventional MINFLUX's,the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.

关 键 词：SCANNING EXCITATION stabilization 

分 类 号：O4[理学—物理]