GANscan:continuous scanning microscopy using deep learning deblurring  

作　　者：Michael John Fanous Gabriel Popescu 

机构地区：[1]Quantitative Light Imaging Laboratory,Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign,Urbana,IL 61801,USA [2]Department of Bioengineering,University of Illinois at Urbana-Champaign,306 N.Wright Street,Urbana,IL 61801,USA [3]Department of Electrical and Computer Engineering,University of Illinois at Urbana-Champaign,306 N.Wright Street,Urbana,IL 61801,USA

出　　处：《Light(Science & Applications)》2022年第10期2378-2387,共10页光（科学与应用)（英文版）

基　　金：This work was funded bythe National Institute of Health(R01CA238191,R01GM129709)。

摘　　要：Most whole slide imaging(WSI)systems today rely on the"stop-and-stare"approach,where,at each field of view,the scanning stage is brought to a complete stop before the camera snaps a picture.This procedure ensures that each image is free of motion blur,which comes at the expense of long acquisition times.In order to speed up the acquisition process,especially for large scanning areas,such as pathology slides,we developed an acquisition method in which the data is acquired continuously while the stage is moving at high speeds.Using generative adversarial networks(GANs),we demonstrate this ultra-fast imaging approach,referred to as GANscan,which restores sharp images from motion blurred videos.GANscan allows us to complete image acquisitions at 30x the throughput of stop-and-stare systems.This method is implemented on a Zeiss Axio Observer Z1 microscope,requires no specialized hardware,and accomplishes successful reconstructions at stage speeds of up to 5000 μm/s.We validate the proposed method by imaging H&E stained tissue sections.Our method not only retrieves crisp images from fast,continuous scans,but also adjusts for defocusing that occurs during scanning within+/-5 μm.Using a consumer GPU,the inference runs at<20 ms/image.

关 键 词：HARDWARE CONTINUOUS IMAGE 

分 类 号：TH742[机械工程—光学工程]