黄柄曲霉ASD胞外酶活性及其性质  

Extracellular Enzyme Activity and Properties of Aspergillus flavus ASD

作　　者：王辉[1] 郑丽娇 于舒怡[1] 刘丽[1] 关天舒[1] 李柏宏[1] 刘长远[1] WANG Hui;ZHENG Li-jiao;YU Shu-yi;LIU Li;LI Bai-hong;LIU Chang-yuan(Institute of Plant Protection,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161;Xinmin Bureau of Agriculture and Rural Affairs,Shenyang,Liaoning 110300)

机构地区：[1]辽宁省农业科学院植物保护研究所,辽宁沈阳110161 [2]新民市农业农村局,辽宁沈阳110300

出　　处：《辽宁农业科学》2022年第6期38-43,共6页Liaoning Agricultural Sciences

基　　金：沈阳市中青年科技创新人才支持计划项目(RC210404)。

摘　　要：为了明确黄柄曲霉ASD对辣椒疫霉菌的生防机理,开展了ASD粗酶液在不同含量致病疫霉菌胁迫下四种胞外酶活性变化,并测定其酸碱稳定性与溶解性。结果表明,胁迫后纤维素酶与木聚糖酶活性显著提高到较高水平,且与底物含量正相关,第3~4 d时酶活性最高;蛋白酶及β-1,3葡聚糖酶活性虽也提高,但幅度小于纤维素酶与木聚糖酶活性变化,且酶活性低。结果说明纤维素酶与木聚糖酶的溶菌作用是ASD抑制辣椒疫霉生长的主要生防机制。此外,ASD粗酶液具有较好的耐酸碱性,在中性与微酸环境中抑菌活性较高;抑菌物质为亲水性物质,但会被乙醇破坏。研究结果可为黄柄曲霉ASD的开发应用提供依据。In order to clarify the biocontrol mechanism of Aspergillus flavipes ASD to Phytophthora capsici,four extracellular enzyme activities,acid-base stability and solubility in different contents of ASD crude enzyme were determined.The results showed that the activities of cellulase and xylanase were significantly increased to a high level under stress,which have positively correlated with substrate content,and showed the highest activities from 3th to 4th day.Although the activities of protease andβ-1,3 glucanase were increased,whereas the variation was less than that of cellulase and xylanase,and the enzyme activities were lower.These results indicated that the bacteriolysis of cellulase and xylanase was the main biocontrol mechanism of ASD to inhibit the development of Phytophthora capsici.In addition,the crude enzyme solution of ASD showed stronger acid and alkaline resistance and high antibacterial activity in neutral and slightly acidic environments.The antimicrobial substance was hydrophilic,but can be destroyed by ethanol.The results can provide a basis for the development and application of ASD in Aspergillus flavoricum.

关 键 词：辣椒疫霉 黄柄曲霉 致病菌胁迫 胞外酶 

分 类 号：S476[农业科学—农业昆虫与害虫防治]