pH值调控柠檬酸污泥厌氧发酵产酸及碳源潜力研究  

Effect of pH on acid production by anaerobic fermentation of citric acid sludge and carbon source potential of fermentation broth

作　　者：孙东霞 周子安 冯志合 胡修玉 祁光霞 董黎明[1] SUN Dong-xia;ZHOU Zi-an;FENG Zhi-he;HU Xiu-yu;QI Guang-xia;DONG Li-ming(Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry,State Environmental Protection Key Laboratory of Food Chain Pollution Control,Beijing Technology and Business University,Beijing 100048,China;China Biotech Fermentation Industry Association,Beijing 100083,China)

机构地区：[1]北京工商大学,中国轻工业清洁生产和资源综合利用重点实验室,国家环境保护食品链污染防治重点实验室,北京100048 [2]中国生物发酵产业协会,北京100083

出　　处：《中国环境科学》2022年第11期5198-5207,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(41861124004)。

摘　　要：以柠檬酸废水厌氧颗粒污泥为接种物,在不同pH值调控条件下开展柠檬酸生产废水剩余活性污泥厌氧发酵产酸研究.通过对发酵液挥发性脂肪酸(VFAs)、有机质、氮磷和污泥脱水性能的分析,探讨了柠檬酸污泥厌氧产酸机制.结果表明,pH≥10的碱性条件更有利于有机质的溶出从而促进VFAs的产生.三维荧光光谱分析发现在恒定pH值下腐殖酸(HA)和富里酸(FA)会大量溶出降低VFAs的产量.初始pH=10是柠檬酸污泥厌氧产酸的最佳pH值,发酵4d的VFAs浓度最高达(6681.47±126.82)mg COD/L,是文献报道中市政污泥产酸量的近2倍,其中乙酸占比49.8%,发酵后产酸功能菌Chloroflexi、Bacteroidota的相对丰度分别由初始的9.52%、10.87%增至16.84%、14.39%,污泥归一化毛细吸水时间(nCST)为(11.34±0.27)s⋅L/g,脱水性能良好,发酵液TP浓度为(20.45±0.33)mg/L.研究表明,利用柠檬酸剩余活性污泥碱性厌氧发酵产酸作为污水处理过程中的外加碳源具有较大潜力.The research of acid production by anaerobic fermentation with different pH control conditions was carried out for the treatment of waste activated sludge from citric acid wastewater,using anaerobic granular sludge of citric acid wastewater as inoculum.The mechanism of anaerobic acid production of citric acid sludge was evaluated by the analysis of volatile fatty acids(VFAs),organic matter,nitrogen and phosphorus contents and sludge dewatering performance.The results showed that the alkaline conditions with pH≥10 were more conducive to the dissolution of organic matter to promote the production of VFAs.It was obvious that humic acid(HA)and fulvic acid(FA)at constant pH conditions would be dissolved in large quantities with Three-dimensional Excitation-Emission-Matrix Spectra analysis,thus reducing the yield of VFAs.The initial pH=10 was the optimum pH value for anaerobic acid production of citric acid sludge,and the VFAs concentration of(6681.47±126.82)mg COD/L for 4 days was the highest,which was nearly 2 times that of municipal sludge acid production reported in the literature,among which acetic acid was 49.8%.After fermentation,the relative abundances of acid-producing functional bacteria Chloroflexi and Bacteroidota increased from initial 9.52%and 10.87%to 16.84%and 14.39%,respectively.The normalized capillary suction time(nCST)value of the final sludge was(11.34±0.27)s·L/g with good dewatering performance,and the TP concentration of fermentation broth was(20.45±0.33)mg/L.Studies have shown that the alkaline anaerobic fermentation of citric acid waste activated sludge to produce acid fermentation broth has a good development potential as an exogenous carbon source in the sewage treatment process.

关 键 词：pH值调控 柠檬酸污泥 碱性厌氧发酵 挥发性脂肪酸 污泥脱水性能 

分 类 号：X705[环境科学与工程—环境工程]