海南雪茄烟叶霉变微生物鉴定及拮抗菌株筛选  

Identification of microorganisms resulted mildew on Hainan cigar tobacco and their antagonistic strains’screening

作　　者：宋嘉宝 王明锋[2] 罗昭标 曹庆梅 夏长剑 侯宁宁 郭林青 马林 李萌 陈德鑫 饶智[2] SONG Jiabao;WANG Mingfeng;LUO Zhaobiao;CAO Qingmei;XIA Changjian;HOU Ningning;GUO Linqing;MA Lin;LI Meng;CHEN Dexin;RAO Zhi(School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China;HongyunHonghe Tobacco(Group)Co.,Ltd.,Kunming 650231,China;Fuzhou Branch of Jiangxi Provincial Tobacco Company,Fuzhou 344000,Jiangxi,China;Hubei Zhongxiang Bioengineering Co.,Ltd.,Yidu 443300,Hubei,China;Haikou Cigar Research Institute,Hainan Provincial Tobacco Company of CNTC,Haikou 571100,China;China Tobacco Sichuan Industrial Co.,Ltd.,Chengdu 610021,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,郑州450001 [2]红云红河烟草(集团)有限责任公司,昆明市650231 [3]江西省烟草公司抚州市公司,江西省抚州市344000 [4]湖北中向生物工程有限公司,湖北省宜昌市443300 [5]中国烟草总公司海南省公司海口雪茄研究所,海口市571100 [6]四川中烟工业有限责任公司,成都市610021

出　　处：《烟草科技》2022年第11期7-13,共7页Tobacco Science & Technology

基　　金：云南中烟工业有限责任公司重点项目“云产雪茄烟叶微生物菌种库构建及功能研究”(JB2022YL02);海南省烟草专卖局(公司)科技项目“提升海南雪茄烟叶可用性生产技术研究与应用”(2020001)。

摘　　要：为明确引起海南雪茄烟叶霉变的微生物种类并筛选其拮抗菌株防治烟叶霉变,分别从海南霉变雪茄烟叶表面分离、纯化得到霉变微生物和拮抗微生物,采用形态学和分子生物学相结合的方法鉴定微生物种类。鉴定和筛选结果表明,引起海南雪茄烟叶霉变的微生物主要为篮状菌属、帚霉属和曲霉属,得到3株霉变菌株分别为Talaromyces funiculosus、Scopulariopsis brevicaulis和Aspergillus occultus。筛选到2株拮抗效果良好的拮抗菌株,分别为枯草芽孢杆菌(Bacillus subtilis)和耐盐芽孢杆菌(Bacillus halotolerans)。引起海南雪茄烟叶霉变的微生物主要为篮状菌属、帚霉属和曲霉属菌株,筛选到的2株芽孢杆菌对致霉菌有良好的拮抗作用,为雪茄烟叶霉变的防治提供了参考。To clarify microorganisms resulted mildew on Hainan cigar tobacco leaves and screen their antagonistic strains to prevent tobacco leaves from mildew,mildew and antagonistic microorganisms were isolated from the surface of Hainan moldy cigar tobacco and purified.Morphological and molecular methods were combined to identify microbial species.The identification and screening results showed that the main microorganisms resulted mildew on Hainan cigar tobacco were Talaromyces funiculosus,Scopulariopsis brevicaulis and Aspergillus occultus.Two antagonistic strains with good antagonistic effects were screened out,namely Bacillus subtilis and Bacillus halotolerans.The main microorganisms resulted mildew on Hainan cigar tobacco were mainly Talaromycetes,Scopularia and Aspergillus strains.The screened two strains of Bacillus had good antagonism to mold,which provided a reference for the prevention and control of mildew on cigar tobacco.

关 键 词：雪茄烟叶 霉变 微生物 拮抗菌株 分离鉴定 

分 类 号：TS414[农业科学—烟草工业]