烟草种子附生细菌群落结构与多样性分析  

Community structure and diversity analyses of adnascent bacteria on tobacco seeds

作　　者：蔡刘体 谢红炼 刘文锋[3] 汪汉成 宋光龙 史彩华[2] 曹亚凡 刘亭亭 孟建玉 CAI Liuti;XIE Honglian;LIU Wenfeng;WANG Hancheng;SONG Guanglong;SHI Caihua;CAO Yafan;LIU Tingting;MENG Jianyu(Guizhou Academy of Tobacco Science,Guiyang 550081,China;College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;Technology Center of China Tobacco Hubei Industrial Co.,Ltd.,Wuhan 430040,China;Tongren Branch of Guizhou Provincial Tobacco Company,Tongren 564100,Guizhou,China;Shanghai Tobacco Group Co.,Ltd.,Shanghai 200082,China)

机构地区：[1]贵州省烟草科学研究院,贵阳市550081 [2]长江大学农学院,湖北省荆州市434025 [3]湖北中烟工业有限责任公司技术中心,武汉市430040 [4]贵州省烟草公司铜仁市公司,贵州省铜仁市564100 [5]上海烟草集团有限责任公司,上海市200082

出　　处：《烟草科技》2022年第11期14-22,共9页Tobacco Science & Technology

基　　金：国家自然科学基金项目“基于转录组学和表型组学的嘧菌酯抑制烟草立枯丝核菌菌核形成机理的研究”(31960550)、“烟草关键生育期叶际微生物群落结构及其时空分布格局和代谢功能研究”(32160522);国家烟草专卖局科技项目“基于叶际微生态的烟区多病害复合侵染机制研究”[110202101048(LS-08)]。

摘　　要：为揭示烟草种子附生细菌的群落结构和多样性,采用分离培养法分析了6个烟草品种(烤烟K326、云烟85、云烟87及红花大金元,雪茄烟301和白肋烟L8)种子可培养附生细菌种类,并采用Illumina高通量测序技术研究了4个品种(K326、云烟85、雪茄烟301和白肋烟L8)种子附生细菌的群落结构与多样性。结果表明:6个品种种子上共分离鉴定出细菌35株,可培养优势附生细菌有假单胞菌(Pseudomonas)、芽胞杆菌(Bacillus)、肠杆菌(Enterobacte)、泛菌(Pantoea)、鞘氨醇单胞菌(Sphingomonas)等菌属。高通量测序结果表明:K326、白肋烟L8和云烟85种子的附生细菌结构上存在共性与差异,共有的优势菌有假单胞菌、泛菌、鞘氨醇杆菌(Sphingobacterium)、肠杆菌、糖芽胞杆菌(Saccharibacillus)、寡养单胞菌(Stenotrophomonas)、根瘤菌(Rhizobium)等菌属,烟草品种附生细菌多样性排序依次为云烟85>K326>L8>301。In order to study the community structure and diversity of adnascent bacteria on tobacco seeds,an isolated culture method was used to analyze the cultivable adnascent bacteria on tobacco seeds of six cultivars(flue-cured tobacco K326,Yunyan 85,Yunyan 87 and Honghuadajingyuan,cigar tobacco 301,and burley tobacco L8).Illumina high-throughput sequencing technology was adopted to investigate the community structure and diversity of the adnascent bacteria on the seeds of K326,Yunyan 85,301,L8.The results showed that a total of 35 bacterial strains were isolated from the seeds of the six tobacco cultivars,and the dominant cultivable adnascent bacteria were Pseudomonas,Bacillus,Enterobacter,Pantoea,Sphingomonas,etc.High-throughput sequencing results indicated that there were similarities and differences in the structure of adnascent bacterial communities of K326,L8 and Yunyan 85 seeds,and the shared dominant bacteria genera were Pseudomonas,Pantoea,Sphingobacterium,Enterobacter,Saccharibacillus,Stenotrophomonas,Rhizobium,etc.The cultivars in the order of seed adnascent bacteria diversity were Yunyan 85>K326>L8>301.

关 键 词：烟草种子 附生细菌 多样性 Illumina高通量测序 群落结构 

分 类 号：S435[农业科学—农业昆虫与害虫防治]