准噶尔双峰驼DGAT1基因外显子8序列生物信息学分析  

Bioinformatics analysis of exon 8 sequence of DGAT1 gene in Junggar Bactrian camels

作　　者：姚怀兵 马强 李玲[1] 闫慧 郝泽林 岳海涛 王玉琢 马英俊 毋状元 汪亮 陈钢粮 杨洁 华实 YAO Huaibing;MA Qiang;LI Ling;YAN Hui;HAO Zelin;YUE Haitao;WANG Yuzhuo;MA Yingjun;WU Zhuangyuan;WANG Liang;CHEN Gangliang;YANG Jie;HUA Shi(College of Life Science and Technology/Xinjiang Provincial Key Laboratory of Biological Resources and Genetic Engineering,Xinjiang University,Urumqi 830046,China;Heilongjiang Provincial Animal Husbandry Station,Harbin 150069,China;Xinjiang Wangyuan Wantuoyuan Co.,Ltd.,Altay 836500,China;Xinjiang Uygur Autonomous Region Altay Bureau of Animal Husbandry and Veterinary,Altay 836500,China;Xinjiang Wangyuan Biological Science and Technology Group Co.,Ltd.,Altay 836500,China;Dairy Research Institute,Xinjiang Academy of Animal Sciences,Urumqi 830000,China)

机构地区：[1]新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐830046 [2]黑龙江省畜牧总站,哈尔滨150069 [3]新疆旺源万驼园有限公司,新疆阿勒泰836500 [4]新疆阿勒泰地区畜牧兽医站,新疆阿勒泰836500 [5]新疆旺源生物科技集团有限公司,新疆阿勒泰836500 [6]新疆畜牧科学院奶业研究所,乌鲁木齐830000

出　　处：《黑龙江畜牧兽医》2022年第21期122-126,141,142,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：新疆维吾尔自治区重点研发专项(2018B01003-1);新疆维吾尔自治区研究生科研创新项目(XJ2019G026)。

摘　　要：为了研究准噶尔双峰驼二酰基甘油酰基转移酶1(diacylgycerol acyltransferase 1,DGAT1)基因外显子8编码区的理化性质与结构,以及为寻找与双峰驼泌乳性状相关的分子遗传标记提供基础资料,试验对准噶尔双峰驼DGAT1基因外显子8进行PCR扩增,利用生物信息学方法对DGAT1蛋白结构、理化性质及信号肽、跨膜位置等进行分析,并比较了准噶尔双峰驼与其他物种DGAT1氨基酸序列的进化关系。结果表明:准噶尔双峰驼DGAT1基因外显子8扩增产物大小为421 bp,编码484个氨基酸,有1个保守域,位于62~468位氨基酸处。DGAT1蛋白属于亲水性蛋白,且为不稳定的非经典分泌蛋白。二级结构中,α-螺旋占48.35%,延伸链占14.46%,β-转角占4.13%,无规则卷曲占33.06%,存在9个蛋白跨膜结构区域。骆驼科的双峰驼、单峰驼和野骆驼的DGAT1蛋白独立成支,与牛DGAT1蛋白的分子进化距离相距较远。准噶尔双峰驼DGAT1基因外显子8序列及蛋白质结构与牛DGAT1蛋白卷曲方式、立体空间结构方面基本一致。说明DGAT1基因在物种间变异不大。In order to study the physicochemical properties and structure of the coding region of exon 8 of diacylgycerol acyltransferase 1(DGAT1) gene in Junggar Bactrian camels, and to provide basic data for finding molecular genetic markers related to lactation traits in Bactrian camels, in the experiment, PCR amplification was performed on exon 8 of the DGAT1 gene of the Junggar Bactrian camels. Bioinformatics methods were used to analyze the structure, physicochemical properties, signal peptide, and transmembrane position of DGAT1 protein in Junggar Bactrian camel, and the evolutionary relationship between the DGAT1 amino acid sequence of Junggar Bactrian camel and other species was compared. The results showed that the amplified product of exon 8 of Junggar Bactrian camel DGAT1 gene was 421 bp in size, encoding for 484 amino acids and there was a conserved domain in the coding region, located at amino acids 62-468. DGAT1 protein was a hydrophilic protein and was an unstable non-classical secreted protein. In the secondary structure, α-helix accounted for 48.35%;extended strand accounted for 14.46%;β-turn accounted for 4.13%, and random coil accounted for 33.06%. There were 9 protein transmembrane structural regions. The DGAT1 proteins of Bactrian camels, Dromedary camels and wild camels of the Camelidae family formed an independent branch, and the molecular evolutionary distance from bovine DGAT1 protein was distant. The sequence and protein structure of exon 8 of the DGAT1 gene of Bactrian camel were basically the same as those of bovine DGAT1 protein in terms of coil mode and three-dimensional spatial structure. The results suggested that the DGAT1 gene did not vary much between species.

关 键 词：双峰驼 DGAT1基因 外显子8 蛋白质 生物信息学分析 

分 类 号：S824[农业科学—畜牧学] Q753[农业科学—畜牧兽医]