Coptis,Pinellia,and Scutellaria as a promising new drug combination for treatment of Helicobacter pylori infection  

作　　者：Zhang Yu Wu-Dong Sheng Xu Yin Yu Bin 

机构地区：[1]Department of Internal Medicine,The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan Province,China [2]Department of Surgery,The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan Province,China

出　　处：《World Journal of Clinical Cases》2022年第34期12500-12514,共15页世界临床病例杂志

基　　金：Supported by the Domestic First-class Construction Disciplines of the Hunan University of Chinese Medicine,No. 2018[03];the Key Scientific Research Project of Hunan Provincial Education Board,No. 20A371;the Key Programs of Administration of Traditional Chinese Medicine of Hunan Province,No. C2022016

摘　　要：BACKGROUND Helicobacter pylori(H.pylori)is the most important infectious agent and plays an important role in the progression of chronic gastritis and the development of gastric cancer.AIM To identify efficient therapeutic agents or strategies that can treat H.pylori infection.METHODS We performed literature analysis,experimental validation,and network pharmacology.First,traditional Chinese medicine(TCM)prescriptions for the treatment of H.pylori infection were obtained from the China National Knowledge Infrastructure,China Biology Medicine,China Science and Technology Journal Database,and WanFang databases.In addition,we conducted a relevant search by Reference Citation Analysis(RCA)(https://www.referencecitationanalysis.com).Next,we used TCM Inheritance Support System V2.5 to identify core drug combinations in the TCM prescriptions.Then,an H.pylori-associated chronic mouse model of gastritis was established.The antibacterial properties and antiinflammatory potential of the core drug combination were evaluated by the rapid urease test,modified Warthin-Starry silver staining,histopathological analysis,and enzyme linked immunosorbent assay.Finally,the active compounds,hub targets,and potential signaling pathways associated with the core drug combination were analyzed by network pharmacology.RESULTS The TCM treatment of H.pylori was mainly based on reinforcing the healthy Qi and eliminating pathogenic factors by simultaneously applying pungent dispersing,bitter descending,cold and warm drugs.The combination of Coptis,Pinellia,and Scutellaria(CPS)was identified as the core drug combination from 207 prescriptions and 168 herbs.This drug combination eradicated H.pylori,alleviated the gastric pathology induced by H.pylori infection,and reduced the expression levels of tumor necrosis factor-α(P=0.024)and interleukin-1β(P=0.001).Moreover,a total of 35 compounds and 2807 targets of CPS were identified using online databases.Nine key compounds(tenaxin I,neobaicalein,norwogonin,skullcapflavone II,baicalein,5,8,2'-trihydro

关 键 词：Coptis Pinellia SCUTELLARIA Anti-Helicobacter pylori Drug combination Traditional Chinese medicine 

分 类 号：R285[医药卫生—中药学]