不同规格鹿茸和鹿角中的硫酸软骨素含量测定及在质量评价中的应用  

Detection of chondroitin sulfate in Cervi Cornu Pantotrichum and Cervi Cornu of different specifications and its application in quality evaluation

作　　者：陆雨顺 张燕停 李志满 陈建波 霍晓慧 曲迪 孙印石[1] LU Yu-shun;ZHANG Yan-ting;LI Zhi-man;CHEN Jian-bo;HUO Xiao-hui;QU Di;SUN Yin-shi(Institute of Special Animal and Plant Sciences,China Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112

出　　处：《中国中药杂志》2022年第19期5203-5208,共6页China Journal of Chinese Materia Medica

基　　金：吉林省科技发展计划项目(20210204065YY)。

摘　　要：为全面比较不同规格鹿茸和鹿角中硫酸软骨素的含量,并探讨硫酸软骨素作为划分鹿茸和鹿角的可行性.该实验采集了15个养殖地的22批不同形态梅花鹿鹿茸(二杠茸、三杈茸)、6批不同养殖地的鹿角和60批不同部位(蜡片、粉片、纱片、骨片)鹿茸饮片,采用高效液相色谱法对鹿角和不同形态、不同部位鹿茸中硫酸软骨素的含量进行测定,利用聚类分析对不同规格鹿茸饮片进行归类.结果表明,鹿茸中含有丰富的硫酸软骨素,其中整支二杠茸中平均质量分数为2.35 mg·g^(-1),整支三杈茸中平均质量分数为1.79 mg·g^(-1),明显高于整支鹿角中硫酸软骨素质量分数0.11 mg-g^(-1).不同规格鹿茸饮片的硫酸软骨素含量也呈现出较大差异,蜡片、粉片、纱片和骨片分别为7.81、8.39、1.33、0.54 mg·g^(-1).基于硫酸软骨素含量的聚类分析显示纱片和骨片可聚为一类且明显区分于蜡片和粉片,实现不同部位鹿茸饮片的良好分离.基于中药质量标志物(Q-marker)选定的五大原则,硫酸软骨素可作为鹿茸和鹿角划分的潜在指标,以及不同规格鹿茸饮片(蜡片、粉片、纱片、骨片)区分的潜在质量标志物.该研究为鹿茸质量评价提供了数据支持.The present study comprehensively compared the content of chondroitin sulfate in Cervi Cornu Pantotrichum(CCP)and Cervi Cornu(CC)of different specifications and explored the feasibility of chondroitin sulfate as an indicator to distinguish between CCP and CC.Twenty-two batches of CCP of different specifications(two-branched velvet antler and three-branched velvet antler)from 15 habitats,CC from 6 habitats,and 60 batches of CCP slices prepared from different parts(wax slices,powder slices,gauze slices,and bone slices)were collected.High-performance liquid chromatography(HPLC)was used to determine chondroitin sulfate content in CCP and CC of different specifications.Cluster analysis was used to classify CCP slices of different specifications.The results showed that CCP contained abundant chondroitin sulfate.The average content of chondroitin sulfate was 2.35 mg·g^(-1) in two-branched velvet antler and 1.79 mg·g^(-1) in three-branched velvet antler,significantly higher than 0.11 mg·g^(-1) in CC.Chondroitin sulfate content in wax slices,powder slices,gauze slices,and bone slices were 7.81,8.39,1.33,and 0.54 mg·g^(-1),respectively.Cluster analysis showed that gauze slices and bone slices could be clustered into one category and distinguished from wax slices and powder slices.CCP slices prepared from different parts could be separated well through chondroitin sulfate content.Based on the five principles of Q-marker selection,chondroitin sulfate can be used as a potential Q-marker for the identification of CCP and CC,as well as a potential quality indicator for CCP slices of different specifications(wax slices,powder slices,gauze slices,and bone slices).This research provides data support for CCP quality evaluation.

关 键 词：鹿茸 鹿角 硫酸软骨素 高效液相色谱法 质量评价 

分 类 号：R284.1[医药卫生—中药学]