甘草多糖对肉鸡生长性能和免疫功能的影响  

Effect of Glycyrrhiza polysaccharide on growth performance and immunologic function of broilers

作　　者：陈文彬 王雪莹[1] 张才[1,2] 马彦博 程源斌[3] 王国永 孔涛 廖成水[1,2] CHEN Wen-bin;WANG Xue-ying;ZHANG Cai;MA Yan-bo;CHENG Yuan-bin;WANG Guo-yong;KONG Tao;LIAO Cheng-shui

机构地区：[1]河南科技大学动物科技学院,河南洛阳471023 [2]河南省动物福利与健康养殖国际联合实验室,河南洛阳471023 [3]洛阳蓝斯利科技有限公司,河南洛阳471023

出　　处：《饲料研究》2022年第18期34-40,共7页Feed Research

基　　金：国家自然科学基金项目(项目编号:31872537);河南省自然科学基金项目(项目编号:182300410087);河南省科学普及基金项目(项目编号:183400410012)。

摘　　要：试验旨在研究甘草多糖(GCP)对肉鸡生长性能、血清免疫和肠道免疫功能的影响。选择240只体重相近的1日龄健康肉鸡(公雏),随机分为4组,每组6个重复,每个重复10只鸡。对照组肉鸡饲喂基础日粮,试验组分别在基础日粮中添加300(300GCP组)、600(600GCP组)和900 mg/kg(900GCP组)的GCP。试验期42 d。结果显示:与对照组相比,600GCP组肉鸡的1~21日龄平均日增重显著提高(P<0.05)。21日龄时,与对照组相比,600GCP组、900GCP组肉鸡注射植物血凝素(PHA-P)12 h的PHA刺激指数显著升高(P<0.05);600GCP组肉鸡血清白细胞介素-6(IL-6)含量显著降低(P<0.05),空肠黏膜分泌型免疫球蛋白A(sIgA)含量显著升高(P<0.05)。42日龄时,与对照组相比,各试验组肉鸡血清IL-6含量显著降低(P<0.05),600GCP组肉鸡血清白细胞介素-2(IL-2)含量显著升高(P<0.05)。21日龄时,300GCP组肉鸡脾脏中IL-2表达量显著高于对照组(P<0.05);42日龄时,600GCP组肉鸡脾脏中IL-2表达量显著高于对照组(P<0.05)。研究表明,日粮添加GCP可在一定程度上提高肉鸡的生长性能,改善血清和肠道免疫状态,促进脾脏中免疫相关基因的表达,提高肉鸡免疫力,添加浓度以600 mg/kg为宜。The study was to investigate the effects of Glycyrrhiza polysaccharides(GCP)on growth performance,serum immune and intestinal immune function of broilers.A total of 240 male broilers with similar body weight at one-day-old were randomly divided into four groups,with six replicates in each group and ten broilers in each replicate.The broilers in control group were fed with basal diet,experimental groups were supplemented with 300(group 300GCP)、600(group 600GCP)and 900 mg/kg(group 900GCP)GCP,respectively.The experiment lasted for 42 d.The results showed that average daily gain of broilers at 1~21 d in group 600GCP was significantly increased(P<0.05).On 21 d,compared with control group,the PHA stimulation index of broilers injected with PHA-P for 12 h in group 600GCP and group 900GCP were significantly higher than that in control group(P<0.05).The serum IL-6 content of broiler chickens in group 600GCP was significantly decreased(P<0.05),and the jejunal mucosal sIgA content was significantly increased(P<0.05).On 42 d,compared with control group,the serum IL-6 content of broilers in each test group were significantly decreased(P<0.05),and the serum IL-2 content of broiler chickens in group 600GCP was significantly increased(P<0.05).On 21 d,the expression of IL-2 in spleen of group 300GCP was significantly higher than that of control group(P<0.05).On 42 d,the expression of IL-2 in spleen of broilers in group 600GCP was significantly higher than that in control group(P<0.05).The study indicates that the supplementation of Glycyrrhiza polysaccharide in diet of broilers can improve the growth performance,serum and intestinal immune status,promote the expression of some immune-related genes in spleen,and improve the immunity of broilers to a certain extent.The GCP optimal supplemental concentration is 600 mg/kg.

关 键 词：甘草多糖 肉鸡 生长性能 免疫功能 

分 类 号：S831[农业科学—畜牧学]