姜黄素对青春前期大鼠睾丸缺血/再灌注Nrf2蛋白表达的影响  

Effects of Curcumin on Nrf2 Expression of Testis With Ischemia-reperfusion Injury in Prepubertal Rats

作　　者：张建华[1] 孟繁亮 朱薇[1] 章学文 王俊 陈思思[1] Zhang Jianhua;Meng Fanliang;Zhu Wei;Zhang Xuewen;Wang Jun;Chen Sisi(Huzhou 3rd People's Hospital,Huzhou 313000,Zhejiang,China)

机构地区：[1]湖州市第三人民医院,浙江湖州313000

出　　处：《中国男科学杂志》2022年第4期53-56,73,共5页Chinese Journal of Andrology

基　　金：浙江省湖州市自然科学资金项目(编号:2019YZ05)。

摘　　要：目的探究姜黄素对青春前期大鼠单侧睾丸扭转复位后核因子E2相关因子-2(Nrf2)表达的影响。方法32只4周龄健康雄性SD大鼠,随机分为假手术组、生理盐水组、姜黄素单次给药组和姜黄素连续给药组,每组8只,建立左侧睾丸扭转复位动物模型(720°,2h)。于术后6周处死大鼠,取左侧睾丸,检测睾丸组织中超氧化物歧化酶(SOD)、丙二醛(MDA)含量及免疫组织化学法测定胞质、胞核Nrf2蛋白表达,计算Nrf2蛋白核转位率。结果睾丸缺血/再灌注后,睾丸组织SOD活性明显降低,MDA含量显著增加。姜黄素单次给药组和连续给药组SOD活性显著增加,MDA含量明显降低(P<0.05);睾丸缺血/再灌注后,睾丸组织Nrf2蛋白核转位率显著升高;姜黄素连续应用组Nrf2蛋白核转位率比生理盐水组显著升高(P<0.05)。结论姜黄素通过激活缺血/再灌注后睾丸Nrf2/ARE信号通路,保护抗氧化酶SOD活性和抑制脂质过氧化,从而对青春前期大鼠睾丸缺血/再灌注有明显的远期保护作用,且连续给药明显优于单次给药。Objective To investigate the effect of curcumin on nuclear factor erythroid 2-related factor 2(Nrf2)expression in testicular tissues of prepubertal rats with unilateral testicular torsion.Methods Total of 32 four-week-old healthy male SD rats were randomly divided into four groups including sham operation group,saline group,curcumin single-dose group,and curcumin successive group,and 8 rats in each group.The animal model of left testicular torsion reduction(720°for 2h)was first established,and then were treated with medication.After six weeks of surgery,all the rats were executed and their left testes were collected immediately for detecting the activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA),and the protein expression of Nrf2 and its nuclear translocation rate.Results The activity of SOD was decreased and the content of MDA was increased when testicular tissue suffered from ischemia-reperfusion injury.The activity of SOD was increased and the content of MDA was decreased in curcumin single-does and successive groups(P<0.05).The nuclear translocation rate of Nrf2 was increased when testicular tissue suffered from ischemia-reperfusion injury.The nuclear translocation rate of Nrf2 in the curcumin successive group increased more significantly than that in the curcumin single-dose group(P<0.05).Conclusion Curcumin has an obvious long-term protective effect on testis with ischemia-reperfusion injury through activating the signal pathway of Nrf2/ARE,protecting the activity of SOD and inhibiting lipid peroxidation.The protective effect of the curcumin successive group is better than that of the curcumin single-does group.

关 键 词：睾丸扭转 姜黄素 Nrf2蛋白 抗氧化酶 再灌注损伤 

分 类 号：R285.5[医药卫生—中药学] R697.24[医药卫生—中医学]