云南微小扇头蜱线粒体基因组全序列测定与分析  

Complete mitochondrial genome sequence of Rhipicephalus microplus

作　　者：刘雅芳 陈彬 芦新焱 李光华 杜春红[2] 姜丹丹 杨兴 LIU Ya-fang;CHEN Bin;LU Xin-yan;LI Guang-hua;DU Chun-hong;JIANG Dan-dan;YANG Xing(School of Basic Medicine,Dali University,Dali 671000,China;Yunnan Institute for Endemic Diseases Control and Prevention,Dali 671000,China;School of Public Health,Dali University,Dali 671000,China)

机构地区：[1]大理大学基础医学院,大理671000 [2]云南省地方病防治所,大理671000 [3]大理大学公共卫生学院,大理671000

出　　处：《中国寄生虫学与寄生虫病杂志》2022年第5期677-681,共5页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(81760607,U2002219);云南省自然科学基金(2017FD139);云南省教育厅科学研究基金(2022J0687)。

摘　　要：2017年9月采用布旗法在云南省临沧市耿马县采集游离的微小扇头蜱,提取蜱DNA,采用通用引物PCR扩增微小扇头蜱的16S rRNA、 12S rRNA、 nad1、 cox3基因片段,采用长PCR扩增上述4个基因区的长片段,产物纯化后采用步移法测序,拼接获得线粒体基因组全序列。结果显示,线粒体基因组全长14 763 bp,包含13个蛋白质编码基因、 2个r RNA基因、 22个t RNA和1个长度为264 bp的非编码区(D-Loop区),碱基组成为A 38.66%、 G 9.02%、 C 11.46%、 T 40.87%,(A+T) 79.53%。蛋白质编码基因起始密码子均为ATN;除COX2、 cox3、 NAD5使用不完整T作为终止密码子外,其余基因终止密码子均为TAA或TAG;基因重叠区有12处共95 bp,长度在1~24 bp;基因间隔区有18处共433 bp,长度在1~311 bp。22个t RNA中,除tRNA、tRNA、 tRNA以外均能形成典型的三叶草结构。Rhipicephalus microplus was collected by the cloth flag method in Yunnan Province in September2017 and was used for DNA extraction. Four long fragments were amplified by long PCR using the primers designed based on 16S r RNA, 12S r RNA, nad1 and cox3 gene sequences. The fragments were sequenced by conserved primer-walking. The complete mitochondrial genome sequence was obtained by splicing. The length of the mitochondrial genome(mtDNA) of Rhipicephalus microplus is 14 763 bp. It contained 13 protein-coding genes, 2rRNA genes, 22 tRNA genes and a non-coding control region of 264 bp(D-Loop region). The base composition was A 38.66%, G 9.02%, C 11.46%, T 40.87%. A + T content was 79.53%. The starting codon of protein-coding genes was ATN. The stop codons were TAA or TAG, except for cox2, cox3, nad5, which used incomplete T as the stop codon. The complete mitochondrial genome have 12 gene overlaps. The length ranges from 1-24 bp. The length of 18 total intergenic regions was 433 bp ranging from 1-311 bp. A total of 22 transferring RNA were found, all of which were typical cloverleaf structures except for tRNA, tRNA, tRNA.

关 键 词：微小扇头蜱 线粒体基因组 序列分析 

分 类 号：R384.41[医药卫生—医学寄生虫学]