2016-2017年中国部分地区TTSuV2流行情况调查与遗传进化分析  

Epidemiology and Genetic Evolution Analysis of TTSuV2 in Some Regions of China from 2015 to 2016

作　　者：王德海 陈文文 朱金海[1] 李春秋[1] 李鹏[1] 孙东波[1] Wang Dehai;Chen Wenwen;Zhu Jinhai;Li Chunqiu;Li Peng;Sun Dongbo(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2022年第6期45-55,76,共12页journal of heilongjiang bayi agricultural university

基　　金：科研团队支持计划猪病防制创新团队(TDJH201804);黑龙江省博士后科研启动基金项目(LBH-Q16188)。

摘　　要：为了解2016-2017年中国地区TTSuV2的流行情况以及流行毒株的遗传演化情况,对中国东北、华中、华东、华北、华南、西南、西北7个地区采集的325份仔猪腹泻样品进行PCR检测,并对其中阳性样品的基因组高变区进行克隆与遗传演化分析。结果显示:TTSuV2阳性样品总数为92份,总阳性率为28.31%;成功克隆出目的基因31条,均属于Kappatorquevirus属的TTSuV k2a基因型,且分布于Group1和Group2。基于全长目的基因同源性分析结果显示,31株TTSuV2型鉴定毒株之间的核苷酸同源性为87.5%~99.7%。UTR核苷酸序列比对结果显示,所有鉴定毒株的核苷酸突变位点主要集中在第51~230 nt。ORF2蛋白序列比对结果显示,所有鉴定毒株的氨基酸突变位点主要集中在第39~49 aa;ORF1蛋白序列比对结果显示,氨基酸突变位点主要集中在第19~50 aa。重组分析结果显示,共存在1株潜在重组毒株TTSuV/515。ORF2基因选择压力分析结果显示,3个密码子位点受到正选择压力,4个密码子位点受到负选择压力。In order to investigate the prevalence of Torque Teno sus virus 2(TTSuV2)in some regions of China from 2015 to 2016,325 piglets diarrhoea samples were collected from 7 regions in China(Northeast,Central,East,North,South,Southwest and Northwest).The samples were tested by PCR,and the genomic hypervariable regions of the positive samples were cloned and analyzed for genetic evolution.The results showed that the total number of positive samples of TTSuV2 was 92,and the total positive rate was 28.31%.31 gene sequences were successfully cloned.Phylogenetic tree showed that the TTSuV2 strains identified in this study all belonged to the TTSuV k2a genotype of Kappatorquevirus genus and distributed in Group1 and Group2.Based on the full-length target gene homology analysis,the nucleotide homology of the identified strains was 87.5%-99.7%.The UTR nucleotide seq-uence alignment showed that the mutation sites of all the identified strains were mainly concentrated in 51-230 nt.The ORF2 protein sequence alignment showed that the amino acid mutation sites of all the identified strains were mainly concentrated in 39-49 aa.The alignment of ORF1 protein sequences showed that the amino acid mutation sites were mainly concentrated in 19-50 aa.Recombinant analysis showed that there was only a potential recombinant strain TTSuV/515.The results of ORF2 gene selection stress analysis showed that the three codon sites were in positive selection pressure and the four codon sites were in negative selection pressure.

关 键 词：猪细环病毒2型 流行情况 遗传演化分析 

分 类 号：S858.26[农业科学—临床兽医学]