贝莱斯芽孢杆菌YX-3的筛选鉴定及其抑菌效果  

Screening and Identification of Bacillus velezensis YX-3 and Antagonistic Effect

作　　者：欧阳晓伦 陈昭 窦鑫[1,2] 张洪奇 高亚梅 晏磊[1,2] 邱化蛟 王伟东[1,2] Ouyang Xiaolun;Chen Zhao;Dou Xin;Zhang Hongqi;Gao Yamei;Yan Lei;Qiu Huajiao;Wang Weidong(Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region/College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;Engineering Research Center of Processing and Utilization of Grain By-products,Ministry of Education;Jiangsu Evolutionary Tree Biotechnology Co.,LTD;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences)

机构地区：[1]黑龙江省寒区环境微生物与农业废弃物资源化利用重点实验室/黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]粮食副产物加工与利用教育部工程研究中心 [3]江苏进化树生物科技有限公司 [4]中国农业科学院麻类研究所

出　　处：《黑龙江八一农垦大学学报》2022年第6期64-70,共7页journal of heilongjiang bayi agricultural university

基　　金：黑土地保护与利用科技创新工程专项资助(XDA28030203);国家自然科学基金项目(32170132);黑龙江农垦总局科技攻关项目(HKKY190404);黑龙江八一农垦大学科技创新团队项目(TDJH201809)。

摘　　要：为了分离出对多种植物病害真菌具有生防潜力的拮抗细菌,通过平板法从银杏外种皮中分离纯化到1株细菌YX-3,采用生理生化试验及16S rDNA测序鉴定细菌的分类地位,利用平板对峙法测定菌株YX-3对6种供试植物病原菌的抑制作用。菌株YX-3菌落呈乳白色,边缘光滑,培养5 d后菌落由饱满转变为中心处塌陷,菌体呈杆状,菌体大小约0.5×3.1μm,芽孢大小约0.5×1.0μm,经革兰氏染色结果为阳性,初步确定为芽孢杆菌,经分子生物学鉴定后,其为贝莱斯芽孢杆菌。贝莱斯芽孢杆菌YX-3对灰梨孢菌、禾谷镰刀菌、藤仓镰刀菌、新月弯孢菌、串珠镰刀菌、尖孢镰刀菌6种供试植物病原菌均具有抑制作用,抑制率在60%~69%之间,贝莱斯芽孢杆菌YX-3对藤仓镰刀菌的拮抗作用较强,抑菌率为69.19%±0.24%,对新月弯孢菌的拮抗作用较弱,抑菌率为60.47%±0.09%。贝莱斯芽孢杆菌YX-3对多种植物病原物具有广谱拮抗作用,具有作为生物防治菌剂的潜在价值。In order to isolate and purify antagonistic bacteria with biocontrol potential against a variety of plant disease fungi from the outer seed coat of Ginkgo biloba L,a strain YX-3 was isolated by plate method.The classification status of the bacteria was identified by 16S rDNA sequencing and physiological and biochemical tests.The inhibitory effects of strain YX-3 on six tested plant pathogens were determined by plate antagonistic method.The colony of strain YX-3 was creamy white with smooth edges.After 5 days of culture,the colony changed from full to collapsed in the center,and the cell was rod shaped,about 0.5×3.1μm,while the spore about 0.5×1.0μm.The gram staining results were positive,and it was preliminarily identified as Bacillus,and identified as Bacillus velezensis by molecular biology.Bacillus velezensis YX-3 had an inhibitory effect on six plant pathogens tested,including Pyricularia oryzae,Fusarium graminearum、Fusarium fujikuroi、Curvularia lunata、Fusarium moniliforme、Fusarium oxysporum with an inhibitory rate of 60%-69%.Bacillus velezensis YX-3 had a strong antagonistic effect on Fusarium fujikuroi,with an inhibitory rate of 69.19%±0.24%.The antagonism to Curvularia lunata was weak,and the inhibition rate was 60.47%±0.09%.Bacillus velezensis YX-3 showed broad spectrum antagonism to many plant pathogens,and had potential value as a biological control agent.

关 键 词：银杏 贝莱斯芽孢杆菌 分离鉴定 抑菌效果 

分 类 号：Q-1[生物学]