低温等离子体活化介质诱导真皮间充质干细胞凋亡研究  

Non-thermal Atmospheric Pressure Plasma Induced Apoptosis of Dermal Mesenchymal Stem Cells

作　　者：王媛 毛莹莹 向虹怡 冯耀元 张惠娜 刘宣辰 袁威 贺鑫梅 邢晓娅 韩英浩[1] Wang Yuan;Mao Yingying;Xiang Hongyi;Feng Yaoyuan;Zhang Huina;Liu Xuanchen;Yuan Wei;He Xinmei;Xing Xiaoya;Han Yinghao(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing163319)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2022年第5期78-84,共7页journal of heilongjiang bayi agricultural university

基　　金：黑龙江八一农垦大学研究生创新科研项目(YJSCX2021-Y102)。

摘　　要：旨在探究低温等离子体(NTAPP)处理的PAM对DMSCs活性的影响,阐明PAM促进DMSCs发生细胞凋亡的机制,在体外提取小鼠原代真皮间充质干细胞(DMSCs),并采用流式细胞术检测其表面Marker,油红O和茜素红检测其成脂成骨能力、流式细胞术测定凋亡情况以及活性氧(ROS)水平、蛋白质免疫印迹检测凋亡相关蛋白以及抗氧化蛋白表达水平。结果表明,成功提取DMSCs,并具有成脂成骨分化能力;与未处理组相比,低温等离子体活化介质(PAM)处理后可引起细胞内的ROS水平升高导致细胞凋亡,同时造成凋亡相关蛋白Bax表达水平上升、Bcl2表达水平下降,以及细胞内抗氧化蛋白Prx I、Prx V、GPX表达水平升高。研究表明,一定剂量的PAM能上调细胞内ROS水平,导致DMSCs细胞凋亡水平上升,研究结果可为今后筛选PAM诱导DMSCs增殖的最佳条件提供前期结果,也为丰富低温等离子体在干细胞领域的研究提供理论依据。To explore the effect of PAM treated with ntapp on the activity of DMSCs,and elucidate the mechanism of PAM promoting apoptosis of DMSCs,primary mouse dermal mesenchymal stem cells(DMSCs)were extracted in vitro,and their surface markers were detected by flow cytometry,the ability of adipogenesis and osteogenesis were detected by oil red O and alizarin red,and the apoptosis and ROS level were determined by flow cytometry.Western blot was used to detect the expression of apoptosis related proteins and antioxidant proteins.The results showed that DMSCs were successfully extracted and had the ability of adipogenic and osteogenic differentiation;Compared with the untreated group,PAM treatment increased the level of ROS and induced apoptosis,increased the expression level of Bax and decreased the expression level of Bcl2,and increased the expression levels of Prx I、Prx V、GPX.It showed that a certain dose of PAM could up regulate the level of ROS in cells,leading to the rise of apoptosis level of DMSCs.The results could provide preliminary results for screening the best conditions for PAM to induce the proliferation of DMSCs in the future,and also provide theoretical basis for enriching the research of low-temperature plasma in the field of stem cells.

关 键 词：真皮间充质干细胞 低温等离子体 活性氧 细胞凋亡 

分 类 号：Q28[生物学—细胞生物学]