胃癌中PTPRS的表达及其对胃癌细胞增殖及迁移侵袭能力的影响  

Expression of PTPRS in gastric cancer and its effects on proliferation,migration and invasion of gastric cancer cells

作　　者：刘歆阳 何梦江[2] 陈巍峰[2] 李全林 周平红 LIU Xin-yang;HE Meng-jiang;CHEN Wei-feng;LI Quan-lin;ZHOU Ping-hong(Endoscopy Center,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Endoscopy Center,Zhongshan Hospital Xiamen Branch,Fudan University,Xiamen 361015,Fujian Province,China)

机构地区：[1]复旦大学附属中山医院内镜中心,上海200032 [2]复旦大学附属中山厦门医院内镜中心,厦门361015

出　　处：《复旦学报（医学版）》2022年第6期924-933,共10页Fudan University Journal of Medical Sciences

基　　金：福建省自然科学基金青创项目(2022J05327);上海市医苑新星青年医学人才培养资助计划[SHWJRS(2021)-99]。

摘　　要：目的探讨受体型酪氨酸磷酸酶S(protein tyrosine phosphatase receptor S,PTPRS)在胃癌中的表达及其与胃癌患者预后的关系,以及PTPRS对胃癌细胞增殖、迁移侵袭能力的影响。方法利用复旦大学附属中山医院的胃癌标本的石蜡切片,免疫组化染色分析PTPRS在胃癌组织中的表达情况,进一步运用卡方检验及生存分析探索PTPRS与胃癌患者临床病理因素和生存时间的关系;运用CCK-8和平板克隆实验检测PTPRS的表达变化对细胞增殖和克隆形成能力的影响;采用划痕实验和Transwell实验检测PTPRS的表达变化对细胞迁移侵袭能力的影响;采用Western blot检测胃癌细胞中相关蛋白表达情况。结果对141例胃癌患者癌组织进行了免疫组化染色,显示PTPRS低表达与不良分化及神经浸润密切相关。生存分析显示PTPRS低表达是胃癌患者生存时间缩短的独立危险因素。对10对胃癌患者的癌组织和癌旁组织进行配qPCR检测,发现癌组织与癌旁组织相比PTPRS表达量显著降低。对胃癌细胞系及正常胃黏膜细胞系的qPCR检测显示,与正常胃黏膜细胞系GES-1相比,胃癌细胞系中PTPRS普遍低表达。在SGC7901及HGC27两种细胞系中用慢病毒转染构建PTPRS表达干扰的稳转株,qPCR验证干扰效率。CCK-8实验发现,PTPRS低表达显著促进胃癌细胞的生长,克隆形成实验表明PTPRS低表达显著促进胃癌细胞克隆形成能力,划痕实验和Transwell实验表明PTPRS低表达显著促进胃癌细胞迁移和侵袭能力。对PTPRS干扰的胃癌细胞系进行EMT相关蛋白的检测,发现PTPRS干扰后细胞出现N-cad、ASMA表达增加等表现。结论PTPRS在胃癌组织中低表达,且与不良预后密切相关。PTPRS低表达可能通过上皮间质转化促进胃癌细胞的增殖、迁移和侵袭。Objective To investigate the expression of protein tyrosine phosphatase receptor S(PTPRS)in gastric cancer and its relationship with the prognosis of gastric cancer patients,as well as the effect of PTPRS on the proliferation,migration and invasion of gastric cancer cells.Methods Immunohistochemical staining was used to analyze the expression of PTPRS in 141 gastric cancer specimen from Zhongshan Hospital,Fudan University,and chi square test and survival analysis were used to explore the relationship between PTPRS and clinicopathological factors as well as survival of gastric cancer patients.CCK-8 and cloning formation assays were used to detect the effect of PTPRS expression on cell proliferation and clone formation.Scratch test and Transwell test were used to detect the effect of PTPRS expression on cell migration and invasion.The expression of protein levels in gastric cancer cells was detected by Western blot.Results Immunohistochemical staining in gastric cancer specimens from 141 cases showed that the low expression of PTPRS was closely related to poor differentiation and nerve infiltration.Survival analysis showed that low expression of PTPRS was an independent risk factor for worse overall survival in patients with gastric cancer.The expression of PTPRS in cancer tissues was significantly lower than that in adjacent tissues in 10 paired tissue samples of gastric cancer patients.The qPCR showed that the expression of PTPRS in gastric cancer cell lines was generally lower than that in normal gastric mucosal cell lines GES-1.In SGC7901 and HGC27 cell lines,stable transgenic strains with PTPRS interference were constructed by lentivirus transfection,and the interference efficiency was verified by qPCR.CCK-8 experiment found that low expression of PTPRS significantly promoted the growth of gastric cancer cells.Clone formation assay showed that low expression of PTPRS significantly promoted the clone formation ability of gastric cancer cells.Scratch experiment and Transwell experiment showed that low expression

关 键 词：受体型酪氨酸磷酸酶S(PTPRS) 胃癌 增殖 迁移侵袭 

分 类 号：R320.67[医药卫生—人体解剖和组织胚胎学]