重组鸡α干扰素生物学活性测定用细胞的筛选  

Study on cell screening for determination of biological activity of recombinant chicken interferon-α

作　　者：王翠[1] 谢彩华[1] 赵雪丽[1] 王淑娟[1] 柴茂 马震原[1] 杨海波[1] 刘影 王东方[1] 闫若潜[1] WANG Cui;XIE Caihua;ZHAO Xueli;WANG Shujuan;CAI Mao;MA Zhengyuan;YANG Haibo;LIU Ying;WANG Dongfang;YAN Ruoqian(Hena Province Animal Disease Prevention and Control Center Henan Province Key laboratory for Monitoring,Eealy Warning Prevention and Control of Animal Diseases,Zhengzhou Henan 450008)

机构地区：[1]河南省动物疫病预防控制中心,河南省重大动物疫病监测预警及防控重点实验室,河南郑州450008

出　　处：《现代牧业》2022年第4期20-24,共5页Modern Animal Husbandry

摘　　要：比较鸡胚成纤维细胞(CEF)和鸡成纤维细胞系(DF-1)两种细胞在重组鸡α干扰素(rChIFN-α)生物学活性测定中的应用效果。选择了三个批次的rChIFN-α在CEF和DF-1上进行生物学活性测定,活性测定结果分别为4.19×10^(7) IU/mL、4.19×10^(7) IU/mL、4.19×10^(7) IU/mL和4.19×10^(7) IU/mL、4.19×10^(7) IU/mL、2.10×10^(7) IU/mL。CEF制备繁琐、成本较高,DF-1比CEF更适合于rChIFN-α生物学活性的测定。The aim of this study was to compare the effects of chicken embryo fibroblast(CEF)and chicken fibroblast(DF-1)in recombinant chicken interferonα(rChIFN-α)biological activity assay.Three batches of rChIFN-αwere selected in biological activity measurement on CEF and DF-1,and the results were 4.19×10^(7) IU/mL,4.19×10^(7) IU/mL,4.19×10^(7) IU/mL and 4.19×10^(7) IU/mL、4.19×10^(7) IU/mL、2.10×10^(7) IU/mL。In view of the cumbersome preparation and high cost of CEF DF-1 are more suitable for rChIFN-αthan CEF in determination of biological activity.

关 键 词：重组鸡α干扰素 鸡胚成纤维细胞 鸡成纤维细胞系 活性 

分 类 号：S859.5[农业科学—临床兽医学]