利用嫩叶外植体建立红腺忍冬组织快繁体系  

Establishment of Tissue Culture and Rapid Propagation System on Lonicera hypoglauca Miq.by Using Young Leaves Explants

作　　者：杨洋 黄旭龙 杨蕾蕾[3] Victor Amoroso 何韩军 YANG Yang;HUANG Xulong;YANG Leilei;Victor Amoroso;HE Hanjun(School of Pharmaceutical Sciences,Hunan University of Medicine,Huaihua Hunan 418000,China;College of Life Sciences,South China Agricultural University,Guangzhou Guangdong 510642,China;Fairylake Botanical Garden,Shenzhen&Chinese Academy of Sciences,Shenzhen Guangdong 518004,China;College of Arts and Sciences,Central Mindanao University,Bukidnon 8710,Philippines)

机构地区：[1]湖南医药学院药学院,湖南怀化418000 [2]华南农业大学生命科学学院,广东广州510642 [3]深圳市中国科学院仙湖植物园,广东深圳518004 [4]College of Arts and Sciences,Central Mindanao University,Bukidnon 8710,Philippines

出　　处：《种子》2022年第11期138-143,共6页Seed

基　　金：深圳市城管科技项目(202007);湖南省教育厅重点项目(18 A 492);广东省自然科学基金面上项目(E 21036)。

摘　　要：本研究以红腺忍冬嫩叶为外植体材料,探讨对应的最适组织快繁体系,为红腺忍冬种苗组织快繁工厂化生产提供新思路。结果表明,外植体最适消毒时间为8 min,最适诱导愈伤组织的培养基配方为:MS+2,4-D 4.0 mg/L+蔗糖30 g/L,平均诱导率为86.67%。适合红腺忍冬愈伤组织分化出芽的培养基配方为:MS+6-BA 1.0 mg/L+NAA 0.10 mg/L+蔗糖30 g/L,平均出芽率为83.33%。适合红腺忍冬分化芽增殖的培养基配方为:MS+6-BA 1.5 mg/L+NAA 0.05 mg/L+蔗糖30 g/L,增殖系数为5.42。适合红腺忍冬诱导生根的培养基配方为:1/2 MS+NAA 0.15 mg/L+活性炭0.3 g/L+蔗糖15 g/L,生根率达91.11%。组培苗在V泥炭土∶V珍珠岩=3∶1的栽培基质中成活率高达100%。The tender leaves of Lonicera hypoglauca Miq.were first used as explants to establish a rapid propagation and regeneration system in vitro in order to provide a new idea for seedling rapid propagation with factory production method.The results showed that the most suitable time for disinfection of the explants was 8 min,and the most suitable medium for induction was MS+2,4-D 4.0 mg/L+sucrose 30 g/L,with an average induced rate of 86.67%.The most suitable medium for induction from callus to differentiation of bud of Lonicera hypoglauca Miq.was MS+6-BA 1.0 mg/L+NAA 0.10 mg/L+sucrose 30 g/L,with an average germination rate of 83.33%.MS+6-BA 1.5 mg/L+NAA 0.05 mg/L+sucrose 30 g/L was the most suitable medium for the multiplication of Lonicera hypoglauca Miq,and the multiplication coefficient was 5.42.The most suitable rooting medium for induction was 1/2 MS+NAA 0.15 mg/L+activated carbon 0.3 g/L+sucrose 15 g/L,with an rooting rate of 91.11%.The survival rate of tissue cultured seedlings in peat soil to perlite equalling 3 to 1 cultivation medium was as high as 100%.

关 键 词：红腺忍冬 嫩叶 组织培养 快繁体系 

分 类 号：S567.79[农业科学—中草药栽培]